Table 1.
Primers used for Real-Time PCR analysis of indicated enzyme transcripts in liver cells.
Figure 1.
The variation of apoptosis rate in Swiss rats under the effect of Cry1Ab protein.
A1: the 30 day feeding Swiss rats in control group. A2: the 90 day feeding Swiss rats in control group. A3: the 30 day feeding Swiss rats in test group. A4: the 90 days feeding Swiss rats in test group. Note: The X coordinate means the percent of dyeing Annexin V cells. The Y coordinate means the percent of dyeing PI cells. The B1 coordinate means the percent of necrosis cells and the late apoptosis cells. The B2 coordinate means the percent of dead cells of mechanical damage. The B3 coordinate means the percent of survived cells. The B4 coordinate means the percent of the early apoptosis cells.
Figure 2.
The variation of calcium ion content in blood lymphocytes of rat under the effect of Cry1Ab protein.
D1:the 30 days feeding Swiss rats in control group. D2: the 90 day feeding Swiss rats in control group. D3: the 30 day feeding Swiss rats in test group. D4: the 90 day feeding Swiss rats in test group.
Figure 3.
Effect of Cry1Ab protein on the blood biochemistry in Swiss rat that feeding for 30 days.
The blood biochemistry indexes including ALB, UREA, TP, TG, TC, AST, ALP, GLU, CREA and ALT were assayed. The red bar represents the control group and the blue bar represents the test group.
Figure 4.
Effect of Cry1Ab protein on the blood biochemistry in Swiss rat that feeding for 90 days.
The blood biochemistry indexes including ALB, UREA, TP, TG, TC, AST, ALP, GLU, CREA and ALT were assayed. The red bar represents the control group and the blue bar represents the test group.
Figure 5.
Effect of Cry1Ab protein on the organ relative weight of Swiss rat.
The organ relative weight was calculated by organ weight/body weight. The body weight of rat was weighed before the rat was dissected and the organ weight of rat was weighed after the rat dissection. A: the organ relative weight in Swiss rat that feeding for 30 days. B: the organ relative weight in Swiss rat that feeding for 90 days.
Figure 6.
Effect of Cry1Ab protein on the enzyme activity of organs in Swiss rat that feeding for 30 days.
The enzyme activities of the organs including brain, kidney, liver, spleen and marrow were assayed. A: the AchE enzyme activity. B: the GSH enzyme activity. C: the CAT enzyme activity. D: the SOD enzyme activity.
Figure 7.
Effect of Cry1Ab protein on the enzyme activity of organs in Swiss rat that feeding for 90 days.
The enzyme activity of these organs including brain, kidney, liver, spleen and marrow was assayed. A described the AchE enzyme activity. B described the GSH enzyme activity. C described the CAT enzyme activity. D described the SOD enzyme activity.
Figure 8.
qPCR analysis of several genes in Swiss rat fed with Cry1Ab protein for 30 and 90 days.
C, control; T, fed with Cry1Ab (test). Columns represent means of three individual measurements and bars indicate standard errors of the mean (SEM). The genes encode AchE, CAT, SOD1, SOD2, SOD3, ALP, AST and ALT respectively.
Table 2.
Effects of transgenic Bt rice on the hematological indicators of rats.