Table 1.
Comparison of short and long training paradigms.
Figure 1.
Barnes maze diagram with quadrants.
The Barnes maze is made up of a circular platform, 48” in diameter, with 20 equally spaced holes around the periphery. The holes are 1” away from the edge and have a 1.75” diameter. The maze is divided into 4 quadrants labeled Target, Positive, Opposite, and Negative with the escape hole being in the center of the Target quadrant.
Figure 2.
Primary latency of training trials shows group differences only in first 4 trials.
A) Primary latency, out of 120 s, for 15-m old wild-type (WT) or triple transgenic (3×Tg) mice receiving 15 training trials (WT n = 32, 3×Tg n = 24). Mean and median values given for comparison. Primary latency over 5 training trials for 15-m old (B; WT n = 15, 3×Tg n = 15) and 4-m old (C; WT n = 14, 3×Tg n = 17) mice. *p<0.05, **p<0.01 compare mean values of WT and 3×Tg.
Figure 3.
Percent holes searched and time in target quadrant show short training can resolve cognitive deficits.
A) Percent holes searched, on probe day, in each of four quadrants by 15-m old wild-type or triple transgenic mice receiving either short or long training. Chance level of holes searched in each quadrant is 25%. B) Time (s) spent in the Target quadrant by all 6 groups of mice. Chance amount of time spent per quadrant is 30 s out of 120 s. C) Percent holes searched in each of four quadrants by WT or TG and 15-m or 4-m old mice receiving short training. *p<0.05, ***p≦0.001 compare WT and 3×Tg.
Figure 4.
Percent of mice being guided to escape hole on training days decreased with training.
Percent of mice being manually guided to the escape hole that did not independently enter in the allotted 2: trials 1–5, 6–10, and 11–15. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compare WT and 3×Tg.