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Figure 1.

Criteria for determining subspecies distinctiveness on Neighbor-joining trees.

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Figure 2.

Neighbor-joining tree showing the K2P distances between Polyura DNA barcodes.

The BOLD Process ID is followed by the sequence length.

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Figure 3.

Neighbor-joining tree showing ‘DNA barcode sharing’ in the genus Charaxes.

The BOLD Process ID or GenBank Accession (GB) is followed by locality.

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Figure 4.

Most distant conspecific and closest interspecific matches for 458 UMKL DNA barcodes when blasted against the full BOLD database.

The DNA barcodes are arranged alphabetically by species name along the horizontal axes. Conspecific similarities below 98% and interspecific similarities above 98% that were associated with different subspecies, misidentified BOLD barcodes, potential cryptic species, short sequence length, barcode sharing and different BINs are highlighted with different coloured data points.

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Figure 5.

Neighbor-joining tree showing K2P distances between Hebomoia glaucippe DNA barcodes.

The BOLD Process ID or GenBank Accession (GB) is followed by locality.

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Figure 6.

Distinctiveness of DNA barcodes from 192 subspecies, representing 80 species of true butterflies.

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Table 1.

Conspecific divergences in DNA barcodes associated with different subspecies designations.

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