Table 1.
Average ranges of clinicalpathological characteristics of 67 patients diagnosed with Breast Cancer.
Table 2.
Frequencies of clinicalpathological characteristics of 67 patients diagnosed with Breast Cancer.
Figure 1.
HER2 detection by immunofluorescence in control arrays, and concordance between HER2 AQUA vs. conventional HER2 IHC.
A: Assignment of the tumor compartment as defined by cytokeratin and DAPI fluorescence in sections with invasive or DCIS lesions. B: Representative immunofluorescent staining of HER2. Original magnification, 20×. C: HER2 signal visualized with high magnification. D: box plots of the distribution of HER2 AQUA versus groups scored by clinical IHC criteria as HER2 negative, 2+ and 3+ evaluated on core needle biopsies (CNBs). E: box plots of the distribution of HER2 AQUA versus groups scored as clinically HER2 negative, 2+ and 3+ evaluated on resection specimens.
Figure 2.
Expression of HER2 in tumor resections compared to CNBs.
AQUA HER2 scores of 63 pairs of CNB (open bars) and tumor resection (filled bars) were assessed. Average FOVs of CNB: average number of FOVs included in analysis of CNB; Average FOVs of Resection: average number of FOVs included in analysis of tumor resection specimens. The noise cut point is marked with a solid line. Each AQUA score represents the mean ±95% CI. The number of pairs where resections are higher, lower than CNBs, or equal to those of CNBs are listed in inset.
Table 3.
HER2 and pTyr1248HER2 AQUA score comparisons between CNBs and resections.
Figure 3.
Fluorescent pTyr1248HER2 IHC in control arrays and antibody validation.
A: Assignment of the tumor compartment as defined by cytokeratin and DAPI detection in sections with invasive or DCIS lesions. B: Representative immunofluorescent detection of pTyr1248HER2. Original magnification, 20×. C: pTyr1248HER2 with high magnification. D: phosphoTyr1248HER2 was detected by Western blot in quiescent cell cultures. β-tubulin was used as a loading control.
Figure 4.
Expression of pTyr1248HER2 in paired specimens.
A: AQUA pTyr1248HER2 scores for 35 pairs of CNB (open bars) and tumor resection (filled bars). Average FOVs of CNB: average number of FOVs included in analysis of core needle biopsies; Average FOVs of Resection: average number of FOVs included in analysis of tumor resection specimens. Each AQUA score represents the mean ±95% CI. The noise cut point is presented as a solid line. The number of pairs where resections are higher than CNBs, lower than CNBs, or equal to those of CNBs are listed in inset.
Figure 5.
Comparison between pTyr1248HER2 and HER2 AQUA.
Scatter plot of HER2 and pTyr1248HER2 AQUA scores (CNBs, open diamonds; Resections, filled triangles). Lines in the graph represent the noise cut points of either HER2 or pTyr1248HER2.