Figure 1.
Progression of huanglongbing (HLB)-related symptoms in ‘Candidatus Liberibacter asiaticus’-inoculated ‘Valencia’ sweet orange seedlings.
A) leaves from control plants 19 weeks after inoculation; B) leaves from HLB-grafted plants 19 weeks after inoculation; C) leaves from control plants 29 weeks after inoculation; D) leaves from HLB-grafted plants 29 weeks after inoculation; E) leaves from control plants 35 weeks after inoculation; F) leaves from HLB-grafted trees 35 weeks after inoculation.
Figure 2.
Progression of huanglongbing (HLB)-related symptoms in ‘Candidatus Liberibacter asiaticus’-inoculated ‘Hamlin’ sweet orange seedlings.
A) leaves from control plants 19 weeks after inoculation; B) leaves from CLas-grafted plants 19 weeks after inoculation; C) leaves from control plants 29 weeks after inoculation; D) leaves from CLas-grafted plants 29 weeks after inoculation; E) leaves from control plants 35 weeks after inoculation; F) leaves from CLas-grafted trees 35 weeks after inoculation.
Figure 3.
Typical APCI-HPLC-MS chromatograms of ‘Hamlin’ and ‘Valencia’ leaf extracts and mass-spectra and UV spectra of unknown 5, 6, 7, and 9.
A) selective ion recording chromatograms of “m/z” specie of 177 in the positive ion mode; B) negative electrospray ionization mass spectra ; C) UV spectra of unknown 5, 6, 7, and 9.
Figure 4.
Principal component analysis of HPLC-MS leaf metabolites from ‘Valencia’ and ‘Hamlin’ trees.
A) score plot of leaf metabolites from ‘Valencia’ trees 3 weeks after inoculation; B) score plot of leaf metabolites from ‘Hamlin’ trees 3 weeks after inoculation; C) score plot of leaf metabolites from ‘Valencia’ trees 38 weeks after inoculation; D) score plot of leaf metabolites from ‘Hamlin’ trees 38 weeks after inoculation.
Figure 5.
Relative amounts versus time (week) of different citrus leaf metabolites from controls and CLas-inoculated trees.
A) unknown 29 in ‘Valencia’ leaves; B) unknown 29 in ‘Hamlin’ leaves; C) unknown 39 in ‘Valencia’ leaves; D) unknown 39 in ‘Hamlin’ leaves.
Figure 6.
Relative amounts versus time (week) of different citrus leaf metabolites from controls and CLas-inoculated trees.
A) unknown 6 in ‘Valencia’ leaves; B) unknown 6 in ‘Hamlin’ leaves; C) unknown 7 in ‘Valencia’ leaves; D) unknown 7 in ‘Hamlin’ leaves; E) unknown 9 in ‘Valencia’ leaves; F) unknown 9 in ‘Hamlin’ leaves.