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Figure 1.

ICC staining of FZD3 in (A) CRC, (B), CAD, (C) colorectal polyp, (D) normal colorectal tissues, (E) positive control and (F) negative control (Original magnification, X400, scale bar = 100 µm).

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Figure 2.

ICC scores of FZD3 in (A) CRC, (B) CAD, (C) colorectal polyp and (D) normal colorectal tissues.

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Figure 3.

ICC scores of FZD3 in various Dukes stage of patients with CRC.

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Figure 4.

ICC staining of FZD3 in (A) a lymph node with CRC metastasis from a Dukes 3 patient and (B) an ovary with CRC metastasis from a Dukes 4 patient (T = tumor, Lym = lymphoid cells, original magnification, X400, scale bar = 100 µm).

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Figure 5.

FZD3 expression in various non-CRC metastatic carcinomas.

(A) Percentage of various non-CRC metastatic carcinomas with FZD3 ICC staining and (B) ICC scores of FZD3 in various non-CRC metastatic carcinomas. (HCC = hepatocellular carcinoma, RCCC = renal clear cell carcinoma, SCC1 = squamous cell carcinoma, LAC = lung adenocarcinoma, LNSCC = lung non-small cell carcinoma, PTC = papillary thyroid carcinoma, BC = breast carcinoma, OCCC = ovary clear cell carcinoma, CNSCC = cervical non-small cell carcinoma, FTSAC = fallopian tube serous adenocarcinoma, TCC = transitional cell carcinoma, SCC2 = small cell carcinoma and CND = carcinoma with neuroendocrine differentiation).

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Figure 6.

ICC staining of FZD3 in (A) a metastatic hepatocellular carcinoma and (B) a metastatic renal clear cell carcinoma (Original magnification, X400, scale bar = 100 µm).

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Figure 7.

ICC scores of FZD3 in (A) adenomas with synchronous carcinomas (AWSC) and (B) pure adenomas.

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Figure 8.

Overall survival analyses for the 1st cohort of 186 Dukes A to D CRC patients (A) stratified by a median FZD3 ICC score of 327, (B) with high and low FZD3 expression and (C) with moderately high and moderately low FZD3 expression.

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