Table 1.
Group 1: unchallenged recipients and development of scrapie in offspring.
Figure 1.
Central Nervous system pathology.
Examples of vacuolation and immunostaining for PrPSc in brain tissue. A. Haematoxylin and Eosin (H&E) staining showing vacuolation in the dorsal motor vagus nucleus of L55 (VRQ/VRQ) had natural scrapie-like disease (x100). B. Strong immunostaining for PrPSc (diagnostic for natural scrapie) with BG4 antibody in the dorsal motor vagus nucleus of L55 (x40). C. Marginal immunostaining for PrPSc (diagnostic for SSBP/1) with BG4 in the dorsal motor vagus in the brain of L111 (VRQ/ARR) (x 100). D. Marginal immunostaining for PrPSc (diagnostic for SSBP/1) with BG4 in the dorsal motor vagus of L101 (VRQ/VRQ) (x100). E. Nil vacuolation (typical for SSBP/1) in an H&E section of the dorsal motor vagus in the brain of L101 (VRQ/VRQ) (x100). F. Marginal immunostaining for PrPSc (diagnostic for SSBP/1) in the brain of L106 (VRQ/VRQ) (x100).
Table 2.
Group 2: resistant recipients challenged with SSBP/1 scrapie and development of scrapie in offspring.
Figure 2.
Strain typing lesion profiles of progeny scrapie cases in mice.
Patterns of severity of vacuolation in nine grey matter areas (medulla, cerebellum, superior colliculus, hypothalamus, thalamus, hippocampus, septum, retrosplenial cortex and cingulate & motor cortex) and three white matter areas (cerebellum, superior cerebral peduncle, basal cerebral peduncle) in brain of tg338 mice challenged with brain from scrapie affected progeny. A: four progeny with lesion profiles similar to SSSBP/1 control. (B) One progeny with lesion profile similar to natural scrapie control.
Table 3.
Susceptible recipients challenged with SSBP/1 and development of scrapie in offspring.
Table 4.
Summary of results, numbers of scrapie cases in each genotype group at risk from scrapie.
Table 5.
Summary of results: numbers of scrapie cases in each genotype group following caesarean derivation or normal birth.
Figure 3.
Immunostaining for PrPSc using BG4 antibody in placental tissue (cotyledon) from recipient ewe R46 (x 40).
Figure 4.
Western blot confirmation of scrapie infection.
Examples of proteinase K treated samples from natural scrapie (lanes 1 & 3) and SSBP/1 (lanes 2 & 4). Loading: Lanes 1 and 2, equivalent to 20 mg starting brain wet weight; Lanes 3 and 4, equivalent to 6.67 mg starting brain wet weight.