Figure 1.
Schematic diagram of FRET assay for evaluating the cleavage efficiency of restriction endonucleases.
Table 1.
Oligonucleotides used in this study.
Figure 2.
Effects of enzyme concentrations on EcoRV cleavage.
(A) Time course plot of fluorescence intensity. (B) Initial velocities affected by enzyme concentrations.
Figure 3.
Effects of 2′-OMeN and PS substitution positions on EcoRV cleavage.
(A) Time course plot of fluorescence intensity of 2′-OMeN substitution. (B) Initial velocities affected by position-dependent 2′-OMeN substitution. (C) Time course plot of fluorescence intensity of PS substitution. (D) Initial velocities affected by position-dependent PS substitution.
Figure 4.
Relative initial velocities (RV) of SpeI cleavage affected by position-dependent substitution.
(A) Effects of 2′-OMeN substitution positions. (B) Effects of PS substitution positions.
Figure 5.
Relative initial velocities (RV) of XbaI cleavage affected by position-dependent substitution.
(A) Effects of 2′-OMeN substitution positions. (B) Effects of PS substitution positions.
Figure 6.
Relative initial velocities (RV) of XhoI cleavage affected by position-dependent substitution.
(A) Effects of 2′-OMeN substitution positions. (B) Effects of PS substitution positions.
Figure 7.
Relative initial velocities (RV) of PstI cleavage affected by position-dependent substitution.
(A) Effects of 2′-OMeN substitution positions. (B) Effects of PS substitution positions.
Figure 8.
Relative initial velocities (RV) of SphI cleavage affected by position-dependent substitution.
(A) Effects of 2′-OMeN substitution positions. (B) Effects of PS substitution positions.
Table 2.
Relative initial cleavage rate of 2′-OMeN modification.
Table 3.
Relative initial cleavage rate of PS modification.