Figure 1.
Sizes of Tru-cut® and wedge liver biopsies.
(A) Intact Tru-cut® biopsy. (B) Transected Tru-cut® biopsy sample used for analysis. (C) Wedge biopsy sample.
Table 1.
Mitochondrial function measurement across 4 different rat liver Tru-cut ®biopsy sizes (18 samples; 6 rats).
Table 2.
Mitochondrial function measurement across 3 different human liver Tru-cut® biopsy mass (18 samples; 6 patients).
Figure 2.
Mitochondrial function stability of rat and human Tru-cut® liver biopsies following different cold storage durations.
Rat and human liver oxidative phosphorylation (A,B), electron transport chain capacity (C,D) and respiratory control ratio (E,F) at 1 hour was significantly higher compared to all other timepoints. Refer to the Table 1 legend for definition of oxidative phosphorylation, electron transport chain capacity and respiratory control ratio. Data are shown as mean ± standard error of mean (n = 3–4). *, P<0.05 (1 hour vs. other timepoints).
Figure 3.
Reproducibility of rat (n = 6) and human (n = 6) Tru-cut® liver biopsies at 1 hour (2 mg).
Oxidative phosphorylation (A,B), electron transport chain capacity (C,D) and respiratory control ratio (E,F) of individual replicates from rat and human liver are shown. Refer to the Table 1 legend for definition of oxidative phosphorylation, electron transport chain capacity and respiratory control ratio. The mean is shown as a horizontal bar. The data indicate that there is tight clustering of replicates around means.
Figure 4.
Utility of Tru-cut® biopsies from matched perfused and ischemic liver tissue samples.
The duration of complete ischemia for rat and human liver biopsies were 20 and 12 minutes (range 6–16 minutes), respectively. Oxidative phosphorylation (A,B), electron transport chain capacity (C,D) and respiratory control ratio (E,F) of ischemic Tru-cut® rat (n = 6) and human (n = 6) liver samples were consistently lower than biopsies from perfused liver samples. Refer to the Table 1 legend for definition of oxidative phosphorylation, electron transport chain capacity and respiratory control ratio. **, P<0.01 vs. perfused samples.
Table 3.
Comparison of Tru-cut® versus permeabilized wedge biopsy sample.