Figure 1.
Photographs of the bursa inseminalis (BI) of female Ae. albopictus inseminated twice as shown by the two distinct masses of semen.
The arrows in A indicate the measurements taken for the bursa surface analysis.
Figure 2.
Copulation of virgin male Ae. albopictus and insemination success.
Percentage of copulations with untreated or sterile males leading to successful (black bars) or unsuccessful (white bars) insemination, in relation to copulation duration.
Table 1.
Duration of copulation, insemination success and spermatheca fill for untreated and sterilized male Ae. albopictus.
Figure 3.
Photographs of the bursa inseminalis (BI) of a virgin female Ae. albopictus (A), and inseminated females dissected after 5 min (B), 1 hour (C), and 48 h following insemination (D).
The arrow indicates sperm cells still motile in the BI.
Figure 4.
Sperm transfer to the Ae. albopictus female bursa inseminalis (BI): mean (+ SEM) relative BI surface area according to the mating status of the female.
N was 61, 15, 57 and 9, respectively, for females inseminated once by an untreated male or a sterile male, and females inseminated twice in an interval shorter or longer than 40: ANOVA, P<0.05.
Figure 5.
Fertility of female Ae. albopictus mated once with an untreated or sterilized male, or twice at various interval of time with males in untreated-sterilized or sterilized-untreated mating sequences.
Individual fertility of females over multiple gonotrophic cycles.
Figure 6.
Insemination capacity of a male Ae. albopictus mated with several females in rapid succession.
Percentage of copulation success on all trials (A) and percentage of insemination success of all copulations (B) according to order of mating opportunities, for untreated and sterilized males. Various degrees of female insemination are represented by different coloration within the bars. The vertical lines within the graphs divide the four mating periods.