Table 1.
Sequence and name of primers used for tested presence or absence of lqs gene.
Figure 1.
Apotome imaging, 7 days after establishment of L. pneumophila Lens A) MG, B) AG and C) CG, in biofilm pre-formed with four non-Legionella strains during 15 days from water networks.
L. pneumophila expressing Ds-Red marker appears in red and non-Legionella bacteria, marked using Draq5 stain, in green. Pictures are representative of 3 independent experiments.
Figure 2.
Orthogonal views of apotome imaging, 7 days after establishment of L. pneumophila Lens A) MG and B) AG, in biofilm pre-formed with four non-Legionella strains during 15 days from water networks.
L. pneumophila expressing Ds-Red marker appears in red and non-Legionella bacteria, marked using Draq5 stain, in green.
Figure 3.
Apotome imaging, 7 days after establishment of L. pneumophila Lens MG, in biofilm pre-formed with each of the four non-Legionella strains during 15 days from water networks.
Establishment in A) Pseudomonas aeuginosa, B) Flavobacterium breve, C) Aeromonas hydrophila and D) Escherichia coli. L. pneumophila expressing Ds-Red marker appears in red and non-Legionella bacteria, marked using Draq5 stain, in green.
Figure 4.
Apotome imaging after 7 days of biofilm development on a glass surface of L. pneumophila Lens A) MG C) AG and polysaccharides produced by B) MG and D) AG L. pneumophila Lens.
Legionella are in green and polysaccharides in blue. Pictures are representative of 6 independent experiments.
Figure 5.
Alcian blue test results after 249 MG and AG L. pneumophila Lens in Evian mineral water.
Error bars indicate standard deviation from six independent experiments. The p-value is less than 0,0001, this difference is considered to be extremely statistically significant.
Figure 6.
Epifluorescence microscopy observation of L. pneumophila Lens expressing ds-Red marker (in red) immediately after amoebae release.
Picture is representative of 2 independent experiments.
Figure 7.
Apotome imaging after 7 days of biofilm development on a glass surface of a mixture of A) AG L. pneumophila Lens or B) MG L. pneumophila Lens expressing Ds-Red marker (in red) or GFP marker (in green) and polysaccharides (in blue).
Pictures are representative of 3 independent experiments.
Figure 8.
Results of qPCR amplification mip gene from MG and AG L. pneumophila Lens deposited on glass surface in Evian mineral water at T = 0 and T = 7 days.
Error bars indicate standard deviation from three independent experiments. The p-value is equal to 0,5913 for AG L. pneumophila Lens and 0,2709 for MG L. pneumophila Lens. It is considered not to be statistically significant (NS).
Figure 9.
Apotome imaging after 7 days of biofilm development on a glass surface of a mixture of L. pneumophila lens AG expressing ds-Red marker (in red), and MG GFP marker (in green).
Polysaccharides appear in blue. Pictures are representative of 3 independent experiments. Scale bar corresponds to 10 µm.
Figure 10.
Apotome imaging after 7 days of biofilm development on a glass surface of a A) L. pneumophila Lens treated with supernatant of AG L. pneumophila Lens or B) with supernatant of AG L. longbeachae.
Bacteria are in red and polysaccharides appear in blue. Pictures are representative of 3 independent experiments.
Figure 11.
Picture of lqs PCR analysis electrophoresis gel of L. pneumophila Lens and L. longbeachae.
Pictures are representative of 3 independent experiments.