Table 1.
Fluorescence intensity in the test zone of an immunochromatographic test strip at different combinations of antibodies against human IgE.
Figure 1.
Selecting the optimal concentration of anti-IgE-QD.
The figure shows the relationship between fluorescence intensity in the test zone of the immunochromatographic assay and the concentration of anti-IgE-QD conjugate. A standard solution of IgE with a concentration of 200 kU/L was used as a sample.
Figure 2.
Change in fluorescence intensity with analysis time.
The figure shows the relationship between the analysis time and the fluorescence intensity in the test zone of the immunochromatographic test system. A standard solution of IgE with a concentration of 200/L was used as a sample.
Figure 3.
Calibration curves for the determination of total IgE in human serum.
A - Calibration curve of the immunochromatographic assay for total IgE in human serum. The calibration curve for the determination of total IgE in human serum by using the developed immunochromatographic assay (n = 10) was obtained. B - Calibration curve of the ELISA for total IgE in human serum. The calibration curve for the determination of total IgE in human serum by using the Total IgE EIA (n = 8) was obtained.
Table 2.
Estimation of total IgE by immunochromatographic assay and ELISA: mean (n = 8) and standard deviation.
Table 3.
Estimation of total IgE by immunochromatographic assay: mean (n = 10) and standard deviation.
Figure 4.
Correlation between the results of ELISA and immunochromatographic assay.
Estimation of human IgE in the standard solutions by the QD-based immunochromatographic assay is strongly correlated with the results obtained using a commercial ELISA kit (R2 = 0.9989).
Figure 5.
Relationship between sample dilution and fluorescence intensity in the test zone.
A sample containing 100/L of total IgE was used at various dilutions. R2 = 0.9687.
Figure 6.
Eatimation of total IgE in human serum for healthy donors and patients with allergic diseases.
The fluorescence intensity was determined in test area in accordance with total IgE content in probe. A - Estimation of total IgE for 43 healthy donors. B - Estimation of total IgE for 52 patients with allergic diseases.
Table 4.
Comparison of the sigmoidal and exponential equations for determining the concentration of total IgE from the calibration curve.
Figure 7.
Correlation between ELISA and immunochromatographic assay results for samples from 95 human subjects.
The immunochromatographic assay is able to estimate total IgE in the serum of human subjects, with good correlation kit (R2 = 0.9884) to the results of a commercial ELISA.