Figure 1.
CP infection induced inflammation.
C57BL/6 mice were infected intratracheally with 5x105 IFU CP and sacrificed at various time points. A) CP bacterial burden. B) Representative H&E stained lung sections. C) Inflammatory score. D) Lymphoid aggregate score. Data for all experiments shown represent at least two independent experiments (pooled together).
Figure 2.
CP infection induces iBALT formation.
A) Paraffin lung sections from CP infected animals were stained for the presence of T, B, and follicular dendritic cells using anti-CD3, CD20, and CD21 antibodies respectively.
Figure 3.
CP infection induced immune infiltrates and cytokines.
Immune cell counts in the A) BALF and B) lung were assessed at various time points after CP infection by flow cytometry. C) Chemokine production in lung homogenates after infection. D) Cytokine production in lung homogenates after infection. Data for all experiments shown represent at least two independent experiments (pooled together).
Figure 4.
Adoptive transfer of M1 macrophages into CP infected mice induces severe long-term inflammation.
A) M1 and M2 macrophage numbers in lung single cell suspensions after CP infection. B–E) 1×106 M1 or M2 BMM were adoptively transferred into mice 7 days after CP infection (5×105 IFU). Mice were sacrificed 35 days after infection. B) Representative H&E stained lung section. C) Inflammatory and lymphoid aggregate scores. D) BALF cell count and immune cell counts in the lung. E) Cytokines in lung homogenates. Data for all experiments shown represent at least two independent experiments (pooled together). *p<0.05, **p<0.01, ***p<0.001 (Student's t test or One-Way ANOVA).
Figure 5.
Adoptive transfer of M1 macrophages into CP infected mice induces severe fibrosis.
A) Representative pico-sirius red stained lung sections. B) % collagen staining of lung sections. Data for all experiments shown represent at least two independent experiments (pooled together). *p<0.05, **p<0.01, ***p<0.001 (One-Way ANOVA).