Figure 1.
Boxplot representation of R. rickettsii load in A. aureolatum adult females.
The bacterial load in unfed ticks incubated at either 25°C (G1) or 35°C (G2) or fed on dogs (G3) was determined by qPCR using specific primers and a TaqMan probe for gltA. The medians are represented by thicker black lines. Outliers are represented by empty circles. *p<0.001, Wilcoxon test.
Figure 2.
Functional classification of R. rickettsii genes modulated by exposure to a 10°C increase in incubation temperature (A) or to blood feeding (B).
Figure 3.
Location and orientation of genes encoding T4SS components repressed (A) or induced (B) by blood feeding in the R. rickettsii genome.
Black arrows represent genes modulated in two biological replicates and grey arrows genes modulated in only one biological replicate in microarray experiments. The modulation of the ATPase VirB4 (A1G_00815; white arrow) was not observed.