Table 1.
Primer sequences and amplicon lengths of qRT-PCR products of target genes.
Figure 1.
Different developmental stages of Angiostron gylus cantonensis and their hosts.
A, Pomacea canaliculata, as the intermediate host. B, the third stage larvae (L3) (10×10). C, Sprague-Dawley (SD) rats, as the difinitive host. D, female of the fifth stage larvae (FL5)(10×4). E, male of the fifth stage larvae (ML5)(10×4). F, female of the adult (FA)(10×). G, male of the adult (MA)(10×).
Figure 2.
2D-DIGE gels illustrating proteins of five developmental stages of A.C.
In standard conditions, internal control samples containing the proteins of mixture of 5 equal protein samples labeled with Cy2 (blue). Cy3 (green) and Cy5 (red) were alternated. Color picture was the overlapping images.
Figure 3.
2-DG gels of total protein extracts from various stages of A.C.
Bottom: Comassie blue-stained gels illustrating differentially regulated spots in pairwise samples of FA (A), MA (B), FL5(C) and ML5 (D) of A.C, as revealed by quantitative and statistical analyses with DeCyder software.
Figure 4.
2-DG gels of common extracts from FA(A)/FL5 (C) and MA (B)/ML5(D) and L3 of A.C.
Bottom: Comassie blue-stained gels illustrating differentially regulated spots common existence in FA(A)/FL5 (C) and MA(B)/ML5 (D), were examined for pairwise differences to the L3(E).
Table 2.
Uniquely identified protein spots from FA, MA, FL5, ML5 and L3.
Table 3.
Unidentified protein spots match to the EST of A. cantonensis.
Figure 5.
Distribution of Gene Ontology terms (level 2) for protein identified in differential expressed in FA, MA, FL5, ML5 and L3 stage of A.C nematodes.
A: Moledular Function B: Biological Process.
Figure 6.
Quantitative Real-time PCR Analysis of Differentially Expressed Proteins of A.C nematodes.
A: The expression of 609 increased to 6.11 fold in FL5 and 5.58 fold in FA, 4.95 fold in MA (P<0.01), but there was little change in ML5 (P>0.05). B: The expression of 1004 increased to 2.60 fold in FL5 and 2.62 fold in FA, 2.50 fold in MA (P<0.01), but there was little change in ML5 (P>0.05).