Figure 1.
GPR55-deficiency can inhibit the development of EAE in C57BL/6 mice.
Female C57BL/6 GPR55 knockout (n=10 white circles) and heterozygous wildtype littermates (n=5. Filled grey circles) were injected with 200μg MOG35-55 peptide in Freunds adjuvant on day 0 and 7. Neurological signs were scored daily 0-5 scale. The results represent the mean ± SEM daily scores (Table 1A).
Figure 2.
Genetic Background may influence the development of EAE in CB2 receptor knockout mice.
(A) Female C57BL/6/J wildtype (black symbols. n= 9) and C57BL/6.Cnr2tm1Dgen CB2 receptor knockout mice (white symbols n=10) were injected with MOG35-55 peptide in Freunds adjuvant and PTX as co-adjuvant (Table 1B). (B) Male and Female wildtype ABH (black symbols n= 13) or ABH.Cnr2tm1Dgen (Grey symbols. n=12) CB2 receptor knockout mice were injected with spinal cord homogenate in Freunds adjuvant on day 0 & 7 (Table 1D). These were injected with vehicle or 25mg/kg i.p. THC daily from day 10 onwards (Table 2B). Neurological signs were scored daily 0-5 scale. The results represent the mean ± SEM daily scores.
Figure 3.
High doses of tetrahydrocannabinol inhibit autoimmunity in EAE via a CB1 receptor-dependent rather than a CB2 receptor-dependent mechanism.
Wildtype (solid symbol) and CB1 receptor (Cnr1tm1Par. White symbol. Table 2A) and CB2 (Cnr2tm1Dgen. Grey symbol. Table 2B) receptor ABH congenic knockout mice were injected with mouse spinal homogenate in Freunds adjuvant on day 0 and 7. Animals were injected daily i.p. with 20-25mg/kg THC (Diamond symbol) in ethanol:cremophor:phosphate buffered saline (1:1:18) or vehicle (round symbol) in 0.1ml. The results show the incidence of EAE, the mean maximal neurological score for the group ± SEM, score of animals that develop clinical EAE ± SEM and the day of onset of neurological signs. *P<0.05, **P<0.01, ***P<0.001 compared to littermate controls.
Figure 4.
GPR55-deficiency has marginal effects on the development of EAE in ABH mice.
(A, C) Female and (B, D) male ABH wildtype (black circles) or ABH.Gpr55-knockout mice (white circles) were injected with spinal cord homogenate in Freunds adjuvant on day 0 and 7 and a relapse was induced on day 28 post-inoculation. Neurological signs were scored daily 0-5 scale. The results show the disease course during (A, B) the initial acute or (C, D) an induced relapse. The results represent the mean ± SEM daily scores. n = 12-21/group.