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Table 1.

Subject information across subgroups, with means and standard deviations (±std). Group effects were evaluated with parametric and Kruskal-Wallis ANOVA tests (four-group for age, BMI and psychological measures, and two-group for OSA parameters).

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Figure 1.

Cold pressor heart rate responses.

A: 37 OSA and 57 control subjects; B: 6 OSA and 20 control female subjects, and 31 OSA and 37 control male subjects. Ai, Bi: Raw heart rate during the complete sequence. Aii, Bii, Biii: Change in heart rate relative to baseline (group mean ± SE), with time-points of significant increase or decrease relative to baseline within-group, and time-points of between-group differences (RMANOVA, p<0.05). Shaded areas indicate challenge periods.

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Figure 1 Expand

Figure 2.

Cold pressor indices.

A: heart rate increase from baseline to initial peak (i); B: sustained heart rate elevation during mid-challenge period (area-under curve, ii); C: rate of change (slope) during mid-challenge increase (iii). Time trend graph illustrates the mean heart rate of the 57 subjects in the control group, with a simplified representation (thick black line) overlaid on the measured values (gray line). Shaded area indicates challenge period.

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Figure 2 Expand

Table 2.

Statistics for mixed models implementing RMANOVA.

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Table 2 Expand

Table 3.

Indices for each challenge.

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Table 3 Expand

Figure 3.

Hand grip heart rate responses.

A: 37 OSA and 57 control subjects; B: 6 OSA and 20 control female subjects, and 31 OSA and 37 control male subjects. Aii, Bii: Raw heart rate during the complete sequence. Aii, Bii, Biii: Change in heart rate relative to baseline (group mean ± SE) averaged over 4 challenges, with time-points of significant increase or decrease relative to baseline within-group, and time-points of between-group differences (RMANOVA, p<0.05). Gray rectangles (hg) indicate challenge periods.

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Figure 3 Expand

Figure 4.

Hand grip indices.

A: heart rate increase from baseline to initial peak (i); B: sustained heart rate elevation during main challenge period (area-under curve, ii). Time trend graph illustrates the mean heart rate of the 57 subjects in the control group, with a simplified representation (thick black line) overlaid on the measured values (gray line). Shaded area indicates challenge period.

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Figure 4 Expand

Figure 5.

Valsalva maneuver heart rate responses.

A: 37 OSA and 57 control subjects; B: 6 OSA and 20 control female subjects, and 31 OSA and 37 control male subjects. Ai, Bi, Raw heart rate and Aii load pressure during the complete sequence. Aiii,Bii, Biii: change in signals relative to baseline (group mean ± SE) averaged over 4 challenges, with time-points of significant increase or decrease relative to baseline within-group, and time-points of between-group differences (RMANOVA, p<0.05). Shaded areas (Val) indicate challenge periods.

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Figure 5 Expand

Figure 6.

Hand grip indices.

A: tachycardia ratio (TR; peak during i/baseline); B: heart rate increase from baseline to initial peak (i); C: Valsalva ratio (VR; peak during ii/minimum during recovery); D: rate of change (slope) during phase II increase (iii). Time-trend graph illustrates the mean heart rate of the 57 subjects in the control group, with a simplified representation (thick black line) overlaid on the measured values (gray line). Shaded area indicates challenge period.

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Figure 6 Expand