Figure 1.
Algicidal activity of actinobacteria isolates on M. aeruginosa agar plates and fluid M. aeruginosa cultures.
Figure 2.
Hyphae and spores of strain L74.
a and b: light microscope ×500; c: SEM ×6,000; d: SEM ×3,000.
Figure 3.
Phylogenetic tree based on the 16S rRNA gene of strain L74.
The tree was inferred using the neighbor-joining method, and evolutionary distances were computed using the Kimura’s 2-parameter model. Bootstrap values (1,000 replicates) above 50 are shown at each node.
Figure 4.
Algicidal effects of Streptomyces sp. L74 cultures to M. aeruginosa.
Control: M. aeruginosa cultures without addition of Streptomyces sp. L74 cultures; A: M. aeruginosa cultures with addition of Streptomyces sp. L74 cultures; B: M. aeruginosa cultures with addition of Streptomyces sp. L74 cell-free cultures after filtration with 0.22 µm microposous membrane; C: M. aeruginosa cultures with addition of Streptomyces sp. L74 cells after centrifugation and washing with distilled water.
Figure 5.
Effects of pHs (A) and temperatures (B) on algicidal activity of algicidal compounds to M. aeruginosa.
Figure 6.
HPLC and UV absorption spectrum of active fraction from Sephadex G15 column.
Figure 7.
Morphology of algicidal compound B3 in SEM.
Figure 8.
Infrared spectroscopy of algicidal compound B3 packed into potassium bromide.
Figure 9.
Structure of compound B3.
Figure 10.
Effects of algicidal compound B3 to M. aeruginosa.
Growth (A), Superoxide Dismutase (SOD), Catalase (CAT), and Peroxidase (POD) Activity (B) and Malonaldehyde (MDA) concentration (C).