Figure 1.
Bacterial community of leaf midribs of scions.
A) Composition and B) relative abundance of the bacterial Operational Taxonomic Units (OTUs) present in leaf midribs of scions from grapefruit rootstocks grafted with HLB-affected lemon scions treated with ampicillin (Amp), gentamicin (Gm) and water (disease control; CK1). The healthy plants were grafted using Las-free lemon scions (healthy control; CK2).
Figure 2.
Huanglongbing symptoms of the inoculated plants.
Healthy grapefruit rootstocks graft-inoculated with HLB-affected lemon scions treated with ampicillin at a concentration of 1.0 g/L (Amp), gentamicin at a concentration of 100 mg/L (Gm), and a water control (disease control, CK1); The healthy plants were graft-inoculated with Las-free lemon scions (healthy control, CK2).
Table 1.
Ca. L. asiaticus (Las) and its transmission in grapefruit graft-inoculated with Las-infected lemon scions treated with ampicillin at 1.0 g/L (Amp), gentamicin at 100 mg/L (Gm), or water (disease control, CK1) as well as grafted with the Las-free lemon scions (healthy control, CK2).
Figure 3.
Distribution of the bacterial OTUs in response to antibiotic treatments.
In the Venn diagram, the numbers in parentheses represent the number of bacterial OTUs that occurred in each antibiotic treatment [ampicillin (Amp) and gentamicin (Gm)], disease controls (CK1), healthy control (CK2) and their intersections. Pie charts A to G correspond to the appropriately labeled Venn diagram areas (A1 to G1 for the Amp treatment and A2 to G2 for the Gm treatment) and show families that contained over 1% of the total OTUs in each area. In pie charts A to G, the names of the families are followed by their frequencies as a percentage (%).