Figure 1.
Relative expression of LMW-PTP and its fast and slow isoforms on the MDA-MB-435 cell line and knockdowns.
Error bars represent standard deviation (n=4). *p<0.05 compared to KD NT and MDA-MB-435. KD NT – scramble sequences siRNA (control); CI, CII; CIII; CV – knockdown of total LMW-PTP; CIV – knockdown of LMW-PTP slow isoform.
Figure 2.
LMW-PTP activity on MDA-MB-435 cell line and knockdowns.
Error bars represent standard deviation (n=4). *p<0.05 compared to KD NT and MDA-MB-435. KD NT – scramble sequences siRNA (control); CI, CII; CIII; CV – knockdown of total LMW-PTP; CIV – knockdown of LMW-PTP slow isoform.
Figure 3.
Cell proliferation of different clones compared to KD NT (n=6).
p > 0.05 for all comparisons. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.
Figure 4.
Cell migration of LMW-PTP KDs and MDA-MB-435 compared to KD NT.
A-E Representative images of cell migration assay. A: t=0, before migration; B-E: t=24h after migration. B: MDA-MB-435; C:KD NT; D:KD LMW-PTP; E: KD LMW-PTP slow isoform. Error bars represent standard deviation (n=3). *p<0.05 compared to KD NT and MDA-MB-435. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.
Figure 5.
Cell Invasion of LMW-PTP KDs and MDA-MB-435.
Error bars represent standard deviation (n=3). p > 0.05 for all comparisons. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.
Figure 6.
RhoA activation in LMW-PTP KDs and MDA-MB-435.
Error bars represent standard deviation (n=3). *p<0.05 compared to KD NT and MDA-MB-435. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.
Figure 7.
Dephosphorylated EphA2 in LMW-PTP KDs and MDA-MB-435.
Error bars represent standard deviation (n=3). p > 0.05 for all comparisons. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.