Figure 1.
A generalized schematic showing the genome structure and organization of members of genus, Tospovirus, using the type member, Tomato spotted wilt virus as a model.
Figure 2.
Symptoms of Tomato spotted wilt virus (TSWV) on un-inoculated systemic leaves following mechanical inoculation with TSWV (A) tomato leaf at 17 days post inoculation (dpi) (B) tomato leaf at 20 dpi , (C) Nicotiana benthamiana leaf at 17 dpi (D) N. benthamiana leaf at 17 dpi.
Figure 3.
Size distribution of total small RNAs (17-26 nt) in libraries prepared from Tomato spotted wilt virus (TSWV) infected tomato and Nicotiana benthamiana.
Figure 4.
Distribution of vsiRNA sequences matching Tomato spotted wilt virus (TSWV) genome from TSWV infected (A) tomato and (B) Nicotiana benthamiana.
The number of reads obtained for TSWV vsiRNAs (17-26 nt) are shown for both the hosts. Note the difference in Y- axis scale for tomato and N. benthamiana.
Figure 5.
Distribution of vsiRNA sequences matching Large (L), Medium (M) and Small (S) RNA of Tomato spotted wilt virus (TSWV) segments in infected (A) tomato and (B) Nicotiana benthamiana.
The number of reads obtained for TSWV vsiRNAs (17-26 nt) obtained for the three genomic segments are shown for both the hosts. Note the difference in Y- axis scale for tomato and N. benthamiana.
Figure 6.
Percentage of Tomato spotted wilt virus (TSWV)-specific antisense and sense vsiRNAs with respect to the coding frame of the genes encoded by Large (L), Medium (M) and Small (S) RNA segments of the virus in TSWV-infected tomato and Nicotiana benthamiana.
Figure 7.
Relative frequency of 5’ terminal nucleotide of Tomato spotted wilt virus (TSWV) vsiRNAs (21 nt) in infected tomato and N.
benthamiana.
Figure 8.
Hotspots for Tomato spotted wilt virus (TSWV) vsiRNAs in Large (L), Medium (M) and Small (S) RNA segments of TSWV in infected (A) tomato and (B) Nicotiana benthamiana.