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Figure 1.

Phase diagram of the multi-component U-type water-diluted microemulsion system at 25°C.

D82 denotes a dilution line at a surfactant/oil phase ratio = 80/20.

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Table 1.

Melting temperatures of the microemulsion components.

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Table 2.

Thermal behavior of microemulsions along dilution line D82 at different water concentrations measured by DSC.

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Figure 2.

DSC curves of microemulsions containing Tween 20 as the surfactant phase, and GML and ethanol at a 1∶1 weight ratio as the oil phase, along dilution line D82: (a) 0–33%, (b) 35–39%, and (c) 41–90% water.

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Figure 3.

Variation in the content of non-freezable (×), bound (□), interphasal (○) and free water (Δ) as a function of water concentration along dilution line D82.

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Figure 4.

Particle size distributions of the microemulsions along dilution line D82 with various water contents: (a) 9%; (b) 38%; (c) 46%, in correspondence to the FF-TEM observations of (a) w/o, (b) bicontinous, and (c) o/w microstructures.

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Figure 5.

Time exposure viability curves for cultures of approximately 109 cfu/ml E. coli (a) and 108 cfu/ml S. aureus (b) at 1% (v/v) addition to microemulsions with various water contents at 25°C for 24 h.

Error bars are calculated from standard error of the dataset (n = 2).

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Figure 6.

Plot of the calculated killing behavior over a period of 24

Region I denotes the w/o microemulsions (<33% water). Region II denotes the bicontinous microemulsions with 35–39% water. Region III denotes the o/w microemulsions (>39% water).

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