Figure 1.
Comparative distribution of eukaryotic sequences in Great Barrier Reef coral holobionts.
(A) Abundance of sequences belonging taxonomic groups of Eukaryotes in corals using three tag encoded 454 amplicon pyrosequencing assays targeting eukaryote small-subunit rRNA gene assays (Euk1, Euk2, Euk3). Relative position of the universal primer pairs utilised Euk1-3 assays is schematically shown on a complete small-subunit rRNA gene (inset right). (B) Venn diagram of sequence 97% sequence identity clusters shared between corals according to Euk1-3 assays. Number of sequences used to calculate Venn diagram within 97% clusters (>1 sequence) obtained using Euk1-3 assay and DNA indicated on the left (colour coded according to coral DNA). Total number of sequences recovered for each Euk1-3 (number in brackets on the right) and number sequences represented by >1 sequence (n). Coral species Acropora palifera 42a, Montipora digitata 60a, Porites cylindrica 66-2 from Heron Island and Seriatopora hystrix 176-2 from the One Tree Island.
Figure 2.
Phylogenetic tree of apicomplexan N-symbiont from the Great Barrier Reef based on small-subunit rRNA gene sequences.
The tree was inferred using the Maximum Likelihood method based on the Tamura-Nei model with a discrete Gamma distribution (5 categories, alpha parameter = 0.2444). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 30 nucleotide sequences and rooted with Cryptosporidium spp. sequences. GenBank accession is shown on the right of the species name. Bootstrap support inferred from 100 replicates is shown (>50%). All positions with less than 75% site coverage were eliminated. There were a total of 1463 positions in the final dataset. Evolutionary analyses were conducted in MEGA5.1.
Figure 3.
Distribution of bacterial sequences in Great Barrier Reef coral holobionts.
(A) Rarefaction curves with 95% confidence interval for sequences obtained using bacterial tag encoded 454 amplicon pyrosequencing assays targeting small-subunit rRNA gene assays (Bac1). Dominance and Chao-1 are indicated above each graph. (B) Abundance of sequences belonging bacterial phyla. Proportion of sequences belonging to bacterial classes within phylum Proteobacteria (inset). Coral species Acropora palifera 42a, Montipora digitata 60a, Porites cylindrica 66-2 from Heron Island and Seriatopora hystrix 176-2 from the One Tree Island.