Figure 1.
Modified primer used for PCR amplification prior to 454-pyrosequencing.
Pyrosequencing fusion primers were as recommended by the manufacturer (Roche), molecular tags were as recommended by Parameswaran et al. (2007) [45] and New Zealand endemic earthworm group-specific primers were as described in Boyer et al. (2011) [15].
Figure 2.
Breakdown of predated earthworm species.
Proportion of the 8,712 good-quality amplicons from each of 16 different earthworm species based on amplicons obtained after 454-pyrosequencing on 46 snail faecal strings.
Figure 3.
Individual diet composition for 46 Powelliphanta augusta snails.
Percentage of amplicons detected for each earthworm species in each faecal string (n=46).
Figure 4.
Frequency of occurrence of each species of earthworm in the diet of P. augusta.
Proportion of faecal strings (n=35) that contained DNA from each earthworm species.