Figure 1.
Mitochondria were isolated from livers of wild-type mice fed a standard or ethanol diet for 6-8 weeks and analyzed for mitochondrial protein acetylation (A), propionylation (B), butyrylation (C), and succinylation (D) by western blot analysis with an acyllysine-specific antibody; normalized to total mitochondrial content using anti-electron transfer flavoprotein (ETF); n = 4 mice/condition.
Figure 2.
Mitochondrial extracts were isolated from livers of wild-type or SIRT3KO mice fed a standard or ethanol diet for 6-8 weeks and analyzed for total protein acetylation (A), propionylation (B), butyrylation (C), and succinylation (D) by western blot analysis with an acyllysine-specific antibody; normalized to total mitochondrial content using anti-voltage-dependent anion channel (VDAC); n = 4 mice/condition.
Figure 3.
Mitochondria were isolated from livers of wild-type mice fed a standard or ethanol diet for 6-8 weeks and analyzed for mitochondrial SIRT3 (A), SIRT4 (B) and SIRT5 (C) expression; normalized to total mitochondrial content using anti-electron transfer flavoprotein (ETF); n = 4 mice/condition.
Figure 4.
Mitochondria were collected from livers of wild-type mice fed a standard or ethanol diet for 1, 3, or 6 weeks and analyzed for total protein acetylation (A), propionylation (B), and succinylation (C) by western blot analysis with acyllysine-specific antibodies; normalized to total mitochondrial content using anti-voltage-dependent anion channel (VDAC); n = 4 mice/condition.
Figure 5.
Nuclear extracts were isolated from livers of wild-type mice fed a standard or ethanol diet for 6-8 weeks and analyzed for total protein acetylation (A), propionylation (B), butyrylation (C), and succinylation (D) by western blot analysis with an acyllysine-specific antibody; normalized to total nuclear content using anti-lamin A/C; n = 4 mice/condition.
Figure 6.
Proteomic data summary of all known acylated proteins identified in mitochondrial and non-mitochondrial compartments.
Area represents number of proteins identified in this study (red), compared to previous studies (blue), as acetylated (10:1 scaling), propionylated, and succinylated.
Figure 7.
Pathway analyses of acylated proteins.
Unique pathway (in box) and number of acylated proteins identified in that pathway (in parenthesis), divided by major sub-cellular compartments.