Table 1.
List of the strains used in this study.
Table 2.
Physiological analysis of the strains used in this study.
Table 3.
Relationship between quinone concentrations and ArcA phosphorylation.
Figure 1.
Growth phase dependence of relative ubiquinol content (%) and ArcA-P activity (%) for MG1655 (Wild type) aerobic and anaerobic batch conditions.
A) Growth curve for MG1655 on Evan’s medium supplied with 20 mM glucose under batch conditions at 37°C. Light line; trend for OD600 under anaerobic conditions, Dark line; trend for OD600 under aerobic conditions. The data is from a single representative experiment and error bars are indicated for each value based on technical triplicates. B) Relative ubiquinol content (%) for MG1655 on Evan’s medium supplied with 20 mM glucose under batch conditions at 37°C. Light line; trend for ubiquinol content (%) under anaerobic conditions, Dark line; trend for ubiquinol content (%) under aerobic conditions. The data is from a single representative experiment and error bars are indicated for each value based on technical triplicates. C) ArcA-P (% (of total ArcA content)) for MG1655 grown on Evan’s medium supplied with 20 mM glucose under batch conditions at 37°C. Light line; trend for ArcA-P (%) under anaerobic conditions, Dark line; trend for ArcA-P (%) under aerobic conditions. The data is from a single representative experiment and error bars are indicated for each value based on technical triplicates.