Table 1.
Strains used in this study ?.
Figure 1.
Staining profile and purity of polysaccharide material used for analysis.
Alcian blue staining is shown on the top, and silver staining is on the bottom. Lane 1, extract from KK01 using heat; lane 2, extract from KK01 using Tris acetate; lane 3, purified material from KK01 using Tris acetate; lane 4, purified material from KK01pamABCDE using Tris acetate; lane 5, purified material from KK01ctrA using Tris acetate; lane 6, purified material from KK01ctrA using surface PBS extraction; lane 7, purified material from KK01ctrA pamABCDE using Tris acetate. MS = molecular size (in kDa).
Table 2.
Glycosyl composition of extracellular preparations of K. kingae strains used in this study.
Figure 2.
1-D proton NMR spectrum of the polysaccharide.
The integration values show the relative molar ratio of the two polysaccharides, Am and (B–C)n of about 1∶3. For numbering, see Table 3 (C3e and C3a designate the equatorial and axial proton, respectively, of the C-3 methylene group).
Figure 3.
2-D HSQC NMR spectrum of the polysaccharide with assignments of all the signals of the three major residues.
For numbering, see Table 3.
Figure 4.
2-D NOESY NMR spectrum of the polysaccharide.
The sequence-determining correlations are labeled. For numbering, see Table 3.
Table 3.
Complete chemical shift assignment of the isolated polysaccharides.
Figure 5.
Structure of capsule polysaccharide repeating unit (top) and galactan exopolysaccharide repeating unit (bottom).
Figure 6.
Colony morphology of K. kingae strains used in this study.
Strain KK01 (A) and KK01pamABCDE (B) form mucoid colonies consistent with encapsulation. KK01ctrA (C) and KK01ctrA pamABCDE (D) form rough, non-mucoid colonies consistent with loss of encapsulation.