Table 1.
Characteristics of all subjects included in the study.
Table 2.
Blood parameters for hemoglobin, thrombocytes, urea and creatinine of all patients included in the study.
Figure 1.
CD55 and CD59 expression are increased on erythrocytes and leukocytes of former EAHEC-infected patients.
Erythrocytes and leukocytes were incubated with CD45-, CD55- and CD59-specific antibodies and analyzed via flow cytometry. The CD45 marker was used to exclude or include the leukocyte population whereas the leukocyte subsets were distinguished via FSC/SSC-plot. Patients were grouped according to their clinical course into 3 groups with either severe gastrointestinal symptoms (GI, n = 34), HUS without neurological symptoms (HUS, n = 23) and HUS with neurological symptoms only (HUS/N, n = 19). Healthy controls were also screened (HC, n = 12). A Erythrocytes, B Leukocytes, C Granulocytes, D Monocytes, E Lymphocytes. All values are given as mean fluorescence intensity (MFI) ± S.D. ANOVA, following symbols are used to pinpoint significant differences: vs. HC *, vs. GI #. One symbol equals 0.05, two symbols 0.01, three symbols 0.001.
Table 3.
Pearson-Bravais correlation tests between CD55 and CD59 expression and hemoglobin, thrombocytes, urea and creatinine levels of all patients included in the study.
Figure 2.
Plasma separation and ECU do not affect CD55 and CD59 expression on erythrocytes and leukocytes.
Erythrocytes and leukocytes were incubated with CD45-, CD55- and CD59-specific antibodies and analyzed via flow cytometry. The CD45 marker was used to exclude or include the leukocyte population whereas the leukocyte subsets were distinguished via FSC/SSC-plot. HUS patients were grouped according to their therapy into 4 groups with HUS patients who received plasma separation (n = 9), HUS patients who received plasma separation and ECU (n = 14), HUS/N patients who received plasma separation (n = 3) and HUS/N patients who received plasma separation and ECU (n = 16). Healthy controls were also screened (HC, n = 12). A Erythrocytes, B Leukocytes. All values are given as mean fluorescence intensity (MFI) ± S.D. ANOVA, following symbols are used to pinpoint significant differences: vs. HC *. One symbols equals 0.05, two symbols 0.01, three symbols 0.001.
Figure 3.
Stx-2 does not reduce the expression of CD55 and CD59 on erythrocytes and leukocytes.
After incubating erythrocytes and leukocytes in whole blood with 0, 0.1 and 10/ml Shiga toxin 2 for 24 h the expression levels for CD55 and CD59 were analyzed via flow cytometry. Patients were grouped according to their clinical course into 3 groups with GI (n = 4), HUS (n = 4) and HUS/N (n = 4). Healthy controls were also screened (HC, n = 4) A CD55 expression, B CD59 expression on erythrocytes, C CD55 expression, D CD59 expression on leukocytes. All values are given as mean fluorescence intensity (MFI) ± S.D.