Figure 1.
LOX-1 expression induced by native LDL or ox-LDL at different time points.
(A) Native LDL-induced LOX-1 expression. (B) ox-LDL-induced LOX-1 expression. The results were obtained from three independent experiments.
Figure 2.
Ginkgolide B inhibits LOX-1 in ox-LDL-stimulated endothelial cells.
The results were obtained from five independent experiments. #p<0.05, compared with control; *p<0.05, compared with ox-LDL-stimulated cells.
Figure 3.
Ginkgolide B inhibits Akt phosphorylation and ICAM-1 expression in ox-LDL-stimulated endothelial cells.
(A) Ginkgolide B inhibited Akt phosphorylation. #p<0.05, compared with control; *p<0.05, compared with ox-LDL-stimulated cells. (B) Ginkgolide B suppressed ICAM-1 expression. #p<0.05, compared with control; *p<0.05, compared with ox-LDL-stimulated cells. (C) LOX-1 and ICAM-1 expression in Akt1 siRNA -transfected and -nontransfected cells. (D) Analysis of LOX-1 expression with Akt siRNA transfection. The results were obtained from three independent experiments. *p<0.05, difference between Akt siRNA-transfected and -nontransfected cells. (F) Analysis of ICAM-1 expression with Akt siRNA transfection. *p<0.05, difference between Akt siRNA-transfected and -nontransfected cells.
Figure 4.
LY294002 inhibits Akt phosphorylation and LOX-1 and ICAM-1 expression in ox-LDL-stimulated endothelial cells.
(A) LY294002 suppressed Akt phosphorylation. (B) LY294002 diminished LOX-1 expression. (C) LY294002 reduced ICAM-1 expression. The data were obtained from five independent experiments. #p<0.05, compared with control; *p<0.05, compared with ox-LDL-stimulated cells.
Figure 5.
Ginkgolide B and LY294002 inhibit p38MAPK phosphorylation.
(A) Ginkgolide B inhibited p38MAPK phosphorylation. (B) LY294002 reduced p38MAPK phosphorylation. #p<0.05, compared with control; *p<.05, compared with ox-LDL-stimulated cells. The data were obtained from five independent experiments.
Figure 6.
Ginkgolide B increases Sirt1 expression in ox-LDL-stimulated endothelial cells.
(A) Ginkgolide B increased Sirt1 expression. #p<0.05, compared with control; *p<0.05, compared with ox-LDL-stimulated cells. (B) LY294002 had no effect on Sirt1 expression. (C) Quantitative analysis of the level of Sirt2 mRNA by RT-PCR. GADPH was used as an internal reference in the reactive system. (D) Sirt1 siRNA increased LOX-1 and ICAM-1 expression. (E) Analysis of LOX-1 expression in Sirt1 siRNA-transfected cells. *p<0.05, difference between Sirt1 siRNA-transfected and -nontransfected cells. (F) Analysis of ICAM-1 expression in Sirt1 siRNA-transfected cells. *p<0.05, difference between Sirt1 siRNA-transfected and -nontransfected cells. The data were obtained from three independent experiments.
Figure 7.
Ginkgolide B decreases Nrf2 expression without reducing the level of Nrf2 mRNA in ox-LDL-stimulated endothelial cells.
(A) Ginkgolide B downregulated Nrf2 expression. (B) LY294002 recovered Nrf2 expression. The data were obtained from five independent experiments. #p<0.05, compared with control; *p<0.05, compared with ox-LDL-stimulated cells. (C) Quantitative analysis of the level of Nrf2 mRNA by RT-PCR. GADPH was used as an internal reference in the reactive system. The data were obtained from three independent experiments.