Figure 1.
Example of compressed cannabis resin (Hashish) confiscated by Moroccan customs.
Left hand: heated block of Hashish. Right hand: unheated block of Hashish.
Table 1.
Sequences of oligonucleotides used in this study.
Table 2.
DNA yield and purity of isolated DNA from the eight cannabis resin samples using Wagner and adapted CTAB protocol (all DNA were re-dissolve in 100 µl sterile deionised water).
Figure 2.
Agarose gel electrophoresis (1%) of PCR amplification product of THCA synthase gene using the primers (a/b).
(A): Amplification of DNA extracted by the adapted protocol. (B1): Amplification of DNA extracted by Wagner CTAB protocol (cannabis resin). (1–8): Indicated number of samples; (L): 100 bp molecular size ladder; the position of 100 bp, 600 bp and 1600 bp fragments is indicated. (B2): Amplification of DNA extracted by Wagner CTAB protocol (using other tissues of cannabis sativa). (GL): Grind leaves of cannabis sativa. (S): Seeds of cannabis. (R): Rod of cannabis sativa. (M): Mixed rood and leaves. (T): Negative control.
Figure 3.
(A): Agarose gel analysis (0.8%) of extracted gDNA by the adapted protocol. (B) : Agarose gel analysis (0.8%) of extracted gDNA by the Wagner protocol. (1–8): Indicated number of samples; (L): 10 kb molecular size ladder with the position of 500 bp, 1000 bp and 10 kb fragments indicated.