Figure 1.
Oncotic lesions (CWS) in the retinal nerve fiber layer were investigated.
After resolution permanent damage remained to the inner retinal layer.
A: Color photograph of the retina of an HIV-positive patient with non-infectious retinopathy. Cotton wool spots (arrow) are present along the retinal blood vessels.
B: Infrared image of the same retina 18 years after resolution of the cotton wool spot with the arrow pointing to its previous location. The green line represents high-resolution spectral-domain optical coherence tomography (SD-OCT) sectioning through the ischemic spot co-localizing it to an adjacent B-scan.
C: Adjacent B-scan showing all retinal layers. The hyper-reflective layer on the top is the nerve fiber layer, original location of the cotton wool spot, which shows thinning. There is a compensatory shift of outer retinal layers including the outer nuclear layer towards the inner retina (arrowhead).
D: Farnsworth-Munsell diagram showing averaged data for all three groups of eyes. Each group - the group with high CD4, the group with low CD4, and the control group – was significantly different from every other group (p < 0.0001 in each comparison).
Figure 2.
Example of the CWS microperimetric abnormality demonstrating long term-damage.
Left Panel: Microperimetry examination overlaid on color fundus photograph, at the area of the previous cotton wool spot. An intraretinal hemorrhage is present superotemporal to the macula and a CWS is inferior to the optic nerve.
Middle Panel: Enlarged image of microperimetry overlay showing sensitivity values in dB over the area of CWS shows decreased sensitivity (lower numbers in violet) over the area of previous cotton wool spot demonstrating long-term damage.
Right Panel: Color photograph of the retina of the same eye with a dot hemorrhage superotemporal to the macula and fading cotton wool spot.
Figure 3.
Heatmap of gene expression changes between posterior pole retinal punches of HIV-positive (10 samples on the right) and HIV-negative donor eyes (six samples on the left).
Red and blue boxes indicate relative over- and under-expression with respect to a mean level between the two groups. Only genes with regularized t-statistic > 1 have been included. Symbols in blue indicate genes discussed in text. The samples are ordered by the donors’ time of death from left to right in each group.
Figure 4.
Interaction network of genes involved in visual perception using MetaCore™ knowledge base [48].
The color reflects direction and size of regulation in HIV-positive retinae compared to HIV-negative controls. A link signifies interaction, a bullet is binding of protein products, an arrow means regulation of transcription or activation, and a hammer means inhibition or deactivation.
Figure 5.
Abundance of transcript measured by qPCR (relative to a reference gene GAPDH) in retinae of HIV-negative and HIV-positive donors as function of time of death.