Table 1.
Primer sequences for human and mouse amino acid transporters and housekeeping genes.
Figure 1.
Summary of GLUT1, 3, 8 and 9 mRNA expression in control, UABL, Ad-hIGF-1 and Ad-LacZ treated mouse placentas.
ANOVA, Post hoc Tukeys test * p<0.05, n>3 for each treatment
Figure 2.
Representative micrographs of GLUT1 (A,B,C), GLUT3 (D,E,F), GLUT8 (G,H,I), GLUT9a (J,K,L) and GLUT9b (M,N,O) in mouse placental labyrinth in the 3 treatment groups, Sham, UABL and Ad-hIGF-1 respectively.
100× magnification. ST identifies syncytiotrophoblast, * identifies a fetal villous vessel, # identifies a maternal blood sinus. Micrographs are representative of 3 biological replicates for each treatment group.
Figure 3.
Localization of GLUT8 in human placenta.
A) Rabbit IgG control demonstrating no staining in human placenta. B) GLUT8 is expressed in the syncytiotrophoblast (closed arrows) and the villous vessel endothelium (black arrowheads) in normal, term, human placenta.
Figure 4.
GLUT8 mRNA expression is significantly increased following exposure of BeWo cells to Ad-IGF-1 at an MOI of 100∶1 for 48 hours.
ANOVA, Tukeys posthoc test ***p<0.005, n = 6 replicates
Figure 5.
GLUT expression levels in BeWo cells.
A:Representative Western blots of GLUT1, 3, and 8 in BeWo cells treated with control media (C), Ad-hIGF-1 MOI 10∶1 (10), Ad-hIGF-1 MOI 100∶1 (100) for 48 hours. B: Summary of GLUT1, 3, 8 protein expression. ANOVA, Tukeys posthoc test, *p<0.05, ** p<0.01, n>4 replicates for each treatment.
Figure 6.
GLUT1 localization is shown in control cells (A) and Ad-hIGF-1 (B) treated BeWo cells1.
GLUT3 is localized to both the perinuclear region and the cell membrane in both control (C) and Ad-hIGF-1 (D) treated BeWo.GLUT8 protein expression is minimal in control BeWo (E) but increased following exposure to Ad-hIGF-1 (F). Under control conditions GLUT9a remains perinuclear (G) but following exposure to Ad-hIGF-1 -localizes to the cell membrane (H). GLUT9b is distributed throughout the cell membrane in control (I) and Ad-hIGF-1 (J) treated BeWo cells. Images are representative of at least 3 different passages of BeWo cells.