Figure 1.
Unigenes functional annotation results.
(A) Top-hit species distribution for BLASTx matches for yellow horn unigenes using the following order of priority: NR, TrEMBL and Swiss-Prot. (B) E-value distribution of top BLASTx hits for each unigene. (C) Distribution of unigenes in length with BLASTx hits compared with those without hits.
Table 1.
Summary of yellow horn 454 sequencing and assembly.
Table 2.
Functional annotation of yellow horn unigenes in public protein databases.
Figure 2.
Clusters of orthologous groups (COG) classifications of yellow horn unigenes.
Figure 3.
Gene Ontology (GO) categories assigined to the yellow horn unigenes.
Figure 4.
Overview of de novo fatty acid (FA) and triacylglycerol(TAG) biosynthesis pathways.
Indentified enzymes include: ACCase, α-acetyl-CoA carboxylase carboxyl transferase (EC:6.4.1.2); MAT, Malonyl-CoA-ACP transacylase (EC:2.3.1.39); KAS, 3-Ketoacyl ACP synthase (KASII, EC: 2.3.1.179; KAS III, EC: 2.3.1.180); KAR, 3-Ketoacyl ACP reductase (EC:1.1.1.100); HAD, 3R-hydroxymyristoyl ACP dehydrase (EC:4.2.1.-); EAR, enoyl-ACP reductase I (EC:1.3.1.9); FATA/B, fatty acyl-ACP thioesterase A/B (EC:3.1.2.14 3.1.2.-); AAD, acyl-ACP desaturase (EC:1.14.19.2); PCH, palmitoyl-CoA hydrolase (EC:3.1.2.2); ACSL, long-chain acyl-CoA synthetase (EC:6.2.1.3); FAD2/6, Δ12(ω6)-Desaturase (EC:1.14.19.-); FAD3/7/8, Δ15(ω3)-Desaturase (EC:1.14.19.-); GK, glycerol kinase (EC:2.7.1.30); ATS1/GPAT, glycerol-3-phosphate acyltransferase (EC:2.3.1.15); LPAT, lysophosphatidyl acyltransferase (EC:2.3.1.51); PP, phosphatidate phosphatase (EC:3.1.3.4); DGAT1, diacylglycerol O-acyltransferase 1 (EC:2.3.1.20); PDAT1, phospholipid: diacylglycerol acyltransferase 1 (EC:2.3.1.158); LPCAT, lysophosphatidylcholine acyltransferase (EC:2.3.1.23 2.3.1.67); PLA2, Phospholipase A2 (EC:3.1.1.4). Lipid substrates are abbreviated: 16∶0, palmitic acid; 18∶0, stearic acid; 18∶1, oleic acid; 18∶2, linoleic acid.
Table 3.
Enzymes/protein related to FA biosynthesis and metabolism identified by annotation of the yellow horn unigenes.
Table 4.
Enzymes related to TAG biosynthesis and metabolism identified by annotation of the yellow horn unigenes.
Table 5.
Putative transcription factors related to the oil biosynthesis in yellow horn.
Table 6.
Number of SSRs in yellow horn.
Figure 5.
Distribution of putative single nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) in the yellow horn transcriptome.