Figure 1.
Phenotypic characterization of strains selected by direct HIP analysis under Phase I or Phase II conditions.
Detailed legend: Relative biomass after 24 h (white bars) or after 21 days (black bars) for strains identified by direct HIP analysis under Phase I (Panel A) or Phase II (Panel B) conditions. Relative biomass after 24 h was estimated by comparing turbidity of the indicated strains with the control strain (BY4743) in the same batch. It was measured as NTUs with a 2100N Turbidimeter (HASH, Loveland. CO). Relative biomass after 21 days was estimated by measuring OD600 of the homogenized culture and comparing data from the deleted strains with those of the control strain (BY4743) in the same batch. Stars indicate statistically significant differences between BY4743 and the deleted strain.
Figure 2.
Final biomass of BY4743 and auxotrophic strains fermenting natural grape must.
Detailed legend: Final biomass for S. cerevisiae strain BY4743 and homozygous strains deleted for genes conferring auxotrophy to inositol, serine, adenine and lysine after fermentation on natural Malvasía/Viura grape must (white bars) and the same must supplemented with the specific compound for auxotrophy complementation (black bars).
Figure 3.
Phenotypic characterization of strains selected by direct HOP analysis under Phase I or Phase II conditions.
Detailed legend: Relative biomass after 24 h (white bars) or after 21 days (black bars) of strains selected from the top of the direct HOP analysis list under Phase I (Panel A) or Phase II (Panel B) conditions; and strains highlighted in the enrichment analysis for HOP experiments under Phase I conditions shown in table 4 (Panel C). Relative biomass after 24 h was estimated by comparing turbidity of the indicated strains with the control strain (BY4743) in the same batch. It was measured as NTUs with a 2100N Turbidimeter (HASH, Loveland. CO). Relative biomass after 21 days was estimated by measuring OD600 of the homogenized culture and comparing data from the deleted strains with those of the control strain (BY4743) in the same batch. Stars indicate statistically significant differences between BY4743 and the deleted strain.
Figure 4.
Phenotypic characterization of strains selected by inverse HIP analysis under Phase I or Phase II conditions.
Detailed legend: Relative biomass after 24 h (white bars) or after 21 days (black bars) for strains identified in the inverse HIP analysis during Phase I (Panel A) and Phase II (Panel B) fermentation conditions. Relative biomass after 24 h was estimated by comparing turbidity of the indicated strains with the control strain (BY4743) in the same batch. It was measured as NTUs with a 2100N Turbidimeter (HASH, Loveland. CO). Relative biomass after 21 days was estimated by measuring OD600 of the homogenized culture and comparing data from the deleted strains with those of the control strain (BY4743) in the same batch. Stars indicate statistically significant differences between BY4743 and the deleted strain.
Figure 5.
Phenotypic characterization of deletion strains highlighted in Piggott et al [27], as well as HOG1 and HOT1 homozygous deletions (manually selected).
Detailed legend: Relative biomass after 24 h (white bars) or after 21 days (black bars) for strains deleted for HOG1 and HOT1 and strains selected from [27]. Panels A and B: strains selected as showing fitness deficiency. Panels C and D: strains selected as showing fitness advantage. Panels A and C: heterozygous deletions. Panels B and D: homozygous deletions. Relative biomass after 24 h was estimated by comparing turbidity of the indicated strains with the control strain (BY4743) in the same batch. It was measured as NTUs with a 2100N Turbidimeter (HASH, Loveland. CO). Relative biomass after 21 days was estimated by measuring OD600 of the homogenized culture and comparing data from the deleted strains with those of the control strain (BY4743) in the same batch. Stars indicate statistically significant differences between BY4743 and the deleted strain.