Figure 1.
Measurement of systemic inflammatory reaction in mice after CLP compared control.
A. Weight of spleen on day of sacrifice. Septic animals show an increase of weight of spleen on day 10 and 16 after induction of sepsis compared to Sham operated animals (Data is shown as mean ± SD, n = 8). B. Serum level of IL-6 on day of sacrifice. Mice that underwent CLP procedure show a significant increase in IL-6 serum level on day 2, 6 and 10 after surgery compared to control animals (Data is shown as mean ± SD, n = 7). C. Serum level of TNF-α. In correlation to IL-6 level in the blood septic mice show also an augmented level of TNF-α on day 6 and 10 after CLP (Data is shown as mean ± SD, n = 7). * p<0,001, ** p<0,01, *** p<0,05.
Figure 2.
Delay of wound closure and epithelialization in septic animals.
A. Day of wound closure. Animals that underwent CLP procedure exhibit a highly significant delay in day of wound closure (Data is shown as box plots of the median, n = 10). B. Percentage of closed wound area on day 0–16 (measured every second day). Septic mice have a highly significant delay in epithelialization as there is a decrease in percentage of closed wound area from day 2 to day 12 after wounding. (Data is shown as mean ± SD, n = 10). * p<0,001, *** p<0,05.
Figure 3.
Expression of TNF-α and TGF-β in wounds.
A. Percentage of TNFα-positive area on days 2, 6, 10 and 16 after wounding. Septic animals express significant less TNF-α compared to control animals on day 2 and 6 after wounding. (Data are expressed as box plots of the median, n = 8). B. Representative pictures of TNF-α staining at the wound margin captured under 100x magnification of sham and CLP animals on day 2, 6, 10 and 16 after staining for TNF-α. Wound margin is located on the right side. C. Percentage of TGF-β-positive area in epidermis on days 2, 6, 10 and 16. Mice that underwent CLP procedure show a reduced level of TGF-β on day 2. (Data are expressed as box plots of the median, n = 7). D. Representative pictures of TGF-β staining at the wound margin captured under 100x magnification of sham and CLP animals on day 2, 6, 10 and 16 after staining for TGF-β. Wound margin is located on the right side. ** p<0,01; *** p<0,05.
Figure 4.
Expression of MMP7 and TIMP1 in wounds.
A. Percentage of MMP7-positive area on days 2, 6, 10 and 16 after wounding. Septic animals show a significant decrease in MMP7 expression on day 2 and 6 compared to control mice. B. Representative pictures of MMP7 staining at the wound margin captured under 100x magnification of sham and CLP animals on day 2, 6, 10 and 16 after staining for MMP7. Wound margin is located on the right side. C. Percentage of TIMP1-positive area on days 2, 6, 10 and 16. TIMP1 was significantly decreased on day 2 after wounding after CLP. In the later phases of wound repair a significant increase of TIMP1 was observed in septic mice compared to control animals. D. Representative pictures of TIMP1 staining at the wound margin captured under 100x magnification of sham and CLP animals on day 2, 6, 10 and 16 after staining for TIMP1. Wound margin is located on the right side. (Data are expressed as box plots of the median n = 8). * p<0,001, ** p<0,01, * p<0,05.