Figure 1.
Chemical structure of antifungalmycin 702.
Figure 2.
Effect of antifungalmycin 702 and validamycin (the positive control) on inhibition of mycelial growth of R. solani.
Figure 3.
Morphology of hyphae treated with PBS buffer (a) and 3.35 µg/ml antifungalmycin 702 (b) at 28° C for 24 h, 48h, and 72h.
Figure 4.
Scanning electron microscopy (2, 000 X) of R. solani treated with PBS buffer (a) and 3.35 µg/ml antifungalmycin 702 (b). Bar: 10 µm.
Figure 5.
Transmission electron micrographs (20, 000 X) of R. solani cellular organelles treated with PBS buffer (a) and 3.35 µg/ml antifungalmycin 702 (b). Bar: 1 µm.
Figure 6.
Effect of antifungalmycin 702 on chitinase (a) and N-acetyl glucosamine content (b) of R. solani.
Figure 7.
Effect of antifungalmycin 702 on membrane permeability (a), soluble sugar leakage (b), and soluble protein leakage (c) of R. solani.
Figure 8.
Effect of antifungalmycin 702 on ergosterol (a) and malondialdehyde (b) content of R. solani. Asterisk denotes a significant difference (P <0.05) compared with CK (the control, 0 µg/ml antifungalmycin 702).