Table 1.
GO function annotation results of differentially expressed genes.
Table 2.
Top 20 of Statistically significant KEGG pathways in response to vaccination.
Figure 1.
Validation of relative expression between microarray data and RT-qPCR results at 28 days post vaccination.
iNOS2a: inducible nitric oxide synthase 2, itga3b: integrin alpha 3b, STAT5: Signal Transducer and Activator of Transcription 5, IL7R: interleukin 7 receptor, IL22: interleukine 22, Bcl6ab: B-cell CLL/lymphoma 6a, Cdc42l: cell division cycle 42 like, Mycb: myelocytomatosis oncogene b, ABCB8: ATP-binding cassette sub-family B member 8, Cts1a: cathepsin L 1 a.
Table 3.
List of differentially expressed genes related to innate immunity.
Figure 2.
Iron uptake and metabolism were enhanced in the bath-vaccinated zebrafish.
Genes related to putative iron metabolism of intracellular iron and competition for extracellular iron were differentially modulated after vaccination with the live attenuated V. anguillarum. Red: up-regulated, Green: down-regulated, Black: not found to be modulated.
Table 4.
List of differentially expressed genes related to adaptive immunity.
Figure 3.
Putative differentiation pathway of Th17 cells in zebrafish.
The genes related to Th17 cells differentiation and proliferation were modulated after vaccination with the live attenuated V. anguillarum. Red: up-regulated, Black: not found to be modulated.
Figure 4.
Temporal expression profiling of Th17-related genes and integrin β7 during 35 days post bath-vaccination.
The mRNA level of each gene was normalized to that of β-actin. Each bar represents the mean of three biological replicates (3 pools) and error bars represent standard deviation. (*p<0.05).
Figure 5.
Effects of vaccination with the live attenuated V. anguillarum on Wnt signaling pathway.
The genes related to Wnt signaling pathway were modulated after vaccination with the live attenuated V. anguillarum. Red: up-regulated, Green: down-regulated, Black: not found to be modulated. WNT: wingless-type MMTV integration site family, DKK1: Dickkopf homologue 1, LRP5/6: LDL-receptor-related protein 5/6, CK1: casein kinase, GSK3β: glycogen synthase kinase 3β, AXIN: axis inhibition protein, DVL: mammalian homologue of Drosophila dishevelled, APC : adenomatous polyposis coli, PKA: cAMP-dependent protein kinase catalytic subunit alpha, PS-1: presenilin 1, PYGO: legless CREB binding protein, LGS: pygopus CREB binding protein, TCF: T-cell factor, GRG:Groucho, CTBP: C-terminal binding protein, HDAC: histone deacetylases, STBM: VANGL planar cell polarity protein 2, DAAM: dishevelled associated activator of morphogenesis, RHOA: ras homolog family member A, RAC: ras-related C3 botulinum toxin substrate, ROCK: Rho-associated coiled-coil containing protein kinase, JNK: Jun N-terminal kinase, JUN: jun proto-oncogene, PLC: phospholipase C, PKC: protein kinase C, CAMKII: calcium calmodulin mediated kinase II, NFAT: nuclear factor of activated T cells.