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Table 1.

Test substance effective dosage, identification and cost.

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Figure 1.

Image of the output from the ViewPoint software video tracked movement of a single adult zebrafish during exposure to hydrochloric acid (pH 3.0) viewed from above the choice chamber.

The overlain red central line represents the point at which the two laminar flows meet. Hydrochloric acid is present in the lower lane of the image with the direction of flow right to left. Tracking indicates the aversive response to the acid area in the lower half, and preference for the dilution water of the upper lane. The tracking line is represented in different colours to indicate relative speeds of movement. Black representing slow, green moderate, and red fast swimming speeds.

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Table 2.

Summary of MLwiN estimates (± se) of the effects for each of the anaesthetic agents in dilution water versus exposure for the response variables Time, Distance and Speed.

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Figure 2.

Shows the average time (seconds) spent in the exposure (red) and control (blue) lanes for each experiment (± SE, n = 10), ranked by aversion (highest to lowest) for the anaesthetics.

N.B. Although each experimental run was 150

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