Figure 1.
The development period of peanut seeds.
(A) Time courses of oil accumulation in developing peanut seeds. Values are the average of two independent measurements, and the same seed material used. (B) The development period of U12 seeds. (C) The development period of U606 seeds. (D) and (E) A confocal section of a U606 seed at 30-40 DAF and 50-60 DAF and stained with Nile red. (F) and (G) A confocal section of a U12 seed at 30-40 DAF and 50-60 DAF and stained with Nile red. Bars = 25 µm.
Figure 2.
A histogram of unigene ontology classification.
The results are summarized in three main categories: biological processes, cellular components and molecular functions. The right y-axis indicates the number of Unigenes in a particular category. The left y-axis indicates the percentage of a specific category of Unigenes in that main category.
Figure 3.
A histogram of clusters of orthologous groups (COG) classification.
Out of 42,562 nr hits, 14,497 Unigenes have a COG classification within the 25 categories.
(A) RNA processing and modification; (B) Chromatin structure and dynamics; (C) Energy production and conversion; (D) Cell cycle control, cell division, chromosome partitioning; (E) Amino acid transport and metabolism; (F) Nucleotide transport and metabolism; (G) Carbohydrate transport and metabolism; (H) Coenzyme transport and metabolism; (I) Lipid transport and metabolism; (J) Translation, ribosomal structure and biogenesis; (K) Transcription; (L) Replication, recombination and repair; (M) Cell wall/membrane/envelope biogenesis; (N) Cell motility; (O) Posttranslational modification, protein turnover, chaperones; (P) Inorganic ion transport and metabolism; (Q) Secondary metabolites biosynthesis, transport and catabolism; (R) General function prediction only; (S) Function unknown; (T) Signal transduction mechanisms; (U) Intracellular trafficking, secretion, and vesicular transport; (V) Defense mechanisms; (W) Extracellular structures; (Y) Nuclear structure; (Z) Cytoskeleton.
Figure 4.
A comparison of differential unigene expression profiles among the different peanut tissues.
(A) Distribution of Unigenes from mRNA-seq from the different seed tissues; (B) Expression level in 606-3vs 12-3; (C) Expression level in 606-5vs 12-5; (D) The number of upregulated or downregulated Unigenes.
Figure 5.
K-means clustering (A) the expression profile (B) of the Unigenes involved in lipid metabolism.
The nine clusters are presented in B (K1 - K9). The error bars represent the standard deviation.
Figure 6.
The contrapuntal patterns of unigene expression in developing peanut seeds.
Peanut enzymes found in these pathways. The icons beside each enzyme name show the total number of peanut Unigenes corresponding to the enzyme in the seeds. FAB, fatty acid biosynthesis; FAM, fatty acid metabolism; GLM, glycerolipid metabolism.