Figure 1.
Chalimus larvae and shed exuviae.
Chalimus larvae and shed exuviae belonging to the chalimus I/II category (A,B) and the chalimus III/IV category (C,D). The exuviae shown are the actual exuviae shed by the depicted larvae. The total length (TL), cephalothorax length (CL) and cephalothorax width (CW) measurements are shown in A.
Figure 2.
Increase in size of L. salmonis larvae post infection.
Total length (TL) measurements from both experiment 1 and 2 are shown in A. Cephalothorax length (CL) and cephalothorax width (CW) measurements from experiment 2 are shown in B-C. Samples assigned to the chalimus I/II category are shown as circles. Chalimus larvae assigned to the chalimus III/IV are shown as triangles. Preadult 1 are shown as diamonds. Post-incubation measurements are not shown except for preadult I females shown as small diamonds. Color codes: black=sex not determined, blue=male, red=female. Red shaded area= size range of cluster 1, blue shaded area=size range of cluster 2. Frames I-IV represent the sizes for the chalimus I-IV stages as reported by Schram [19] and durations as reported by Johnson and Albright [28] (chalimus IV duration extends to 21 DPI). Size and duration boxes are not shown for CL as no data were reported.
Figure 3.
Cluster analysis and cluster number selection for chalimi.
The proportion of unexplained variability in the dataset as a function of the number of chalimus clusters is shown in A. Total length (TL), cephalothorax length (CL) and cephalothorax width (CW) for experiment 2 (mm) are shown in B. Chalimus larvae assigned to chalimus cluster 1 are shown as diamonds. Chalimus larvae assigned to chalimus cluster 2 are shown as circles. Preadult 1samples are shown as triangles. Color codes: black=sex not determined, blue=male, red=female. Post-incubation measurements are not shown except for preadult I females (red triangles). The assignments to cluster (chalimus cluster 1 and 2) and morphological group (chalimus I/II, chalimus III/IV or preadult category) were congruent for all samples except the individual marked with an arrow.
Figure 4.
Time of molting in incubators for chalimus I/II and chalimus III/IV larvae. The presence of a shed exuvium was used as evidence that a molt had taken place within the past 24 h. Chalimus I/II=black, chalimus III/IV males=blue, chalimus III/IV females=red. DPI= days post infection.
Figure 5.
Size measurements for L. salmonis larvae incubated in hatching wells.
Pre-incubation measurements for incubated samples are shown as blue circles (chalimus I/II category) and red triangles (chalimus III/IV category). The post-incubation measurements are shown as blue triangles (chalimus III-IV category) and red diamonds (preadults). For molted individuals the pre- and post-incubation measurements are connected with a dotted line. Frames I-IV represent the sizes for the chalimus I-IV stages as reported by Schram [19] and durations as reported by Johnson and Albright [28].