Figure 1.
Chemical structure of the colloidal substances employed.
1) Succinylated gelatin chain whose terminal carboxyl group ist deprotonated at physiological pH. 2) Hydroxyethyl starch whose single glucose subunits are additionally hydroxyethylated at carbon atoms two, three and six.
Table 1.
Composition of the five volume replacement solutions Gelafundin, Gelafusal, Geloplasma, Voluven and Volulyte.
Figure 2.
Effect of hemodilution on the current hematocrit.
Rats underwent uniform normovolemic hemodilution to a final hematocrit of 10% with either Gelafundin, Gelafusal or Geloplasma by withdrawal of 3 ml blood per step. An interval of 15 min was left between the single steps. Hematocrit was determined in arterial blood samples immediately before the next withdrawal.
Figure 3.
Influence of different volume replacement solutions on blood pressure during and subsequent to normovolemic hemodilution.
Rats underwent normovolemic hemodilution to a final hematocrit of 10% for 162 min (phase of hemodilution: light grey) with either Gelafundin, Gelafusal or Geloplasma and were subsequently observed for 150 min. For analysis mean arterial blood pressure was monitored.
Figure 4.
Impact of different volume replacement solutions on acid-base parameters during and subsequent to normovolemic hemodilution.
Rats underwent normovolemic hemodilution to a final hematocrit of 10% for 162 min (phase of hemodilution: light grey) with either Gelafundin, Gelafusal, Geloplasma, Voluven or Volulyte and were subsequently observed for 150 min. Parameters were determined in arterial blood samples at the points indicated. * <0.05 compared with the value measured at 3 min, in the respective group. # <0.05 (Voluven vs. Volulyte).
Table 2.
Influence of different volume replacement solutions (Gelafundin, Gelafusal, Geloplasma, Voluven, Volulyte) on lactate and glucose in arterial blood samples during and subsequent to normovolemic hemodilution in rats.
Figure 5.
Effects of different volume replacement solutions on electrolyte parameters during and subsequent to normovolemic hemodilution.
Rats underwent normovolemic hemodilution to a final hematocrit of 10% for 162 min (phase of hemodilution: light grey) with either Gelafundin, Gelafusal, Geloplasma, Voluven or Volulyte and were subsequently observed for 150 min. Electrolytes were determined in arterial blood samples at the points indicated. * <0.05 compared with the value measured at 3 min, in the respective group. # <0.05 (Voluven vs. Volulyte) ** <0.05 (Gelafundin vs. Gelafusal).
Table 3.
Effects of different volume replacement solutions on electrolyte and metabolic parameters as well as pH during and subsequent to normovolemic hemodilution in urine.
Table 4.
Quantification of succinylated gelatin in urine samples taken during and subsequent to normovolemic hemodilution.