Figure 1.
Anther development in fertile and sterile (GMS) Chinese cabbage.
Chinese cabbage flower buds were fixed, embedded in paraffin, and sliced into 8–10 µm transverse sections as described in the Materials and Methods. The bud sections were stained with fast green and the counterstain safranin, and anthers were photographed by bright-field microscopy.
A-D depict anther development in fertile flower buds; E-H depict anther development in sterile flower buds. A and E, microspore mother cell stage; B and F, tetrad stage; C, uninucleate microspore stage; D, mature pollen; G, abnormal tapetal cells; H, abortive pollen.
Figure 2.
Distribution of genes expressed specifically according to genotype.
A, Venn diagram of the distribution of genes expressed specifically according to genotype of Chinese cabbage. B, K-means clustering and graph format of the expression pattern of F- and S-specific genes. Pink colored lines indicate average PI values. The specific genes were classified into four F-specific gene clusters or three S-specific gene clusters by K-means clustering of MeV software (http://www.tm4.org/mev.html). The number in the brackets indicates the gene number of each cluster.
Figure 3.
Expression of genes previously identified in male sterile mutants of Arabidopsis and other Brassica species.
A, Major genes mentioned by Wijeratne et al., 2007. B, Other pollen development-associated genes identified in Arabidopsis. C and D, Late pollen development-associated genes identified in Arabidopsis and Brassica species. Arrows indicate putative GMS-associated genes.
Figure 4.
Hierarchical cluster display of the transcription factors in Chinese cabbage.
The color scale bar shown above the cluster indicates the maximum and minimum brightness values that represent the PI value.
Figure 5.
Hierarchical cluster display of pollen development-associated genes in Chinese cabbage.
The color scale bar shown above the cluster indicates the maximum and minimum brightness values that represent the PI value.