Table 1.
The supplement factors of defined complete culture medium.
Table 2.
Uncomplete culture medium condition.
Figure 1.
Changes of body length (A) and width (B) in third-stage larvae after culture with complete medium that contained 0 to 20% FCS.
All worm body lengths on day 36 after culture with complete medium that contained FCS significantly exceeded those of third-stage larvae before culture. (*p<0.01, **p<0.05).
Table 3.
The development of larvae cultured with complete medium containing 20% FCS.
Figure 2.
Rates of development of third-stage to fourth-stage larvae after 36 days of culture in the complete medium that contained different concentrations of FCS.
The rates of development are determined percentage of fourth-stage larvae numbers of all cultured worms.
Figure 3.
Survival rates of AcL3 after culture with complete and incomplete media that contained 20% FCS.
The survival rates are definitived as 100% on culture day 1 and follow determined as culture day.
Figure 4.
Larvae of A. cantonensis developed in complete medium that contained 20% FCS.
(a) Early third-stage larva. (b) Middle third-stage larva, showing evacuation of gut (arrow). (c) Late third-stage larva, showing expansion and evacuation of gut (arrow). (d) Fourth-stage larva enclosed in sheath (arrow). (e) Anterior end of fourth-stage larva, showing rod-shaped structures retained in sheath (arrow). (f) Exsheathing fourth-stage larva. (g) Exsheathed fourth-stage larva. (h) Comparison of sizes of fourth-stage larva from in vitro culture and third-stage larva of A. cantonensis. Fourth-stage larva on day 36 of culture (lower right-hand side) is significantly larger than third-stage larva (upper left-hand side). Bar = 0.25 mm.
Figure 5.
Comparisons of length (5A) and width (5B) of worms 36 days after culture with complete medium that contained 20% FCS with those of worms from brain of BALB/c mice after infection with A. cantonensis.
Both length and width of worms from brain of BALB/c five days after infection were significantly larger than those of worms cultivated in vitro (*p<0.01).