Table 1.
Clinical, anamnestic and demographic features of the warfarin-treated patients.
Figure 1.
Relationship between the weekly warfarin dose and CYP2C9 (Panel A) or VKORC1 -1639G>A (Panel B) genotypes.
Each box indicates the values from 25° to 75° percentile (interquartile range), the horizontal lines represent the median value of weekly warfarin dose, the maximum length of whisker is 1.5 fold the interquartile range. * p<0.05, ** p<0.001at ANOVA test.
Table 2.
Allele and genotype frequencies of CYP2C9*2, CYP2C9*3, VKORC1 (-1639 G>A, 1173 C>T, 3730 G>A) and CYP4F2 1297G>A polymorphisms obtained in our population and in other Caucasian populations.
Figure 2.
Combined effect of CYP2C9 and VKORC1 -1639 G>A polymorphic genotypes on stable weekly warfarin dose (mg/week).
Each box indicates the values from 25° to 75° percentile (interquartile range), the white lines represent the median value of weekly warfarin dose, the maximum length of whisker is 1.5 fold the interquartile range. In detail below are shown the specific statistical significances for each comparison. CYP2C9 *1/*1+VKORC1 -1639 G/G vs CYP2C9 *1/*1+VKORC1 -1639A/A, p<0.001. CYP2C9 *1/*1+VKORC1 -1639 G/G vs CYP2C9 *1/*1+VKORC1 -1639G/A, p<0.05. CYP2C9 *1/*1+VKORC1 -1639 G/A vs CYP2C9 *1/*1+VKORC1 -1639A/A, p<0.001. CYP2C9 *1/*2+VKORC1 -1639 G/G vs CYP2C9 *1/*2+VKORC1 -1639A/A, p<0.001. CYP2C9 *1/*2+VKORC1 -1639 G/G vs CYP2C9 *1/*2+VKORC1 -1639G/A, p<0.05. CYP2C9 *1/*2+VKORC1 -1639 G/A vs CYP2C9 *1/*2+VKORC1 -1639A/A, p<0.001. CYP2C9 *1/*3+VKORC1 -1639 G/G vs CYP2C9 *1/*3+VKORC1 -1639A/A, p<0.05. CYP2C9 *1/*3+VKORC1 -1639 G/A vs CYP2C9 *1/*3+VKORC1 -1639A/A, p<0.05.
Table 3.
Factors affecting weekly warfarin dose requirements in regression model*.
Figure 3.
Correlation analysis (Pearson coefficient) between our predicted warfarin dosage by Jackknife and that predicted by www.warfarindosing.org (panel A, p<0.001, R2 = 0.805) and www.pharmgkb.org (panel B, p<0.001, R2 = 0.773).
The solid line represent a good correlation between the two doses. Open circles represent individuals for whom no variants in CYP2C9 and VKORC1 -1639 G>A were detected. Gray circles represent individuals with only one polymorphic gene (either VKORC1 -1639 G>A or CYP2C9* 2 or *3). Black circles represent individuals with at least two polymorphic genes.