Figure 1.
The three sampling sites around Ward Hunt Lake are shown in the red boxes. The inset map displays the location of Ward Hunt Island in the Canadian Arctic archipelago. Global positioning system coordinates for the sampling sites: Site 1, 83°05.263’N, 74°10.293’W; Site 2, 83°05.268’N, 74°10.346’W; Site 3, 83°04.887’N, 74°10.491’W.
Table 1.
Characteristics of soil samples.
Table 2.
Sequencing and diversity statisticsa.
Table 3.
Comparison of bacterial diversity of these datasets to selected environments.
Table 4.
Similarity of representative sequences from the 25 most abundant OTUs to sequences in GenBank.
Figure 2.
Principal component analysis of the relationship between 16S rRNA gene sequence datasets.
A) Clustering of datasets based on community membership. B) Clustering of datasets based on community structure.
Figure 3.
Relative abundance of phylum level taxonomic bins in the 16S rRNA gene sequence datasets.
The 10 most abundant phyla across the datasets are displayed. The category rare represents the sum total of all phyla made up of <1% of sequences.
Figure 4.
Correspondence between phyla relative abundance inside and outside of water track samples.
Each phylum detected is represented by a point in the graph and the points are colored based on whether they are more abundant in the inside or outside samples. Error bars represent the standard deviation of the mean across technical replicates (in most cases error bars do not extend outside of the plotted point). Points showing large differences in abundance between inside and outside of the water tracks are labeled with the phylum name. Abbreviations: Acido, Acidobacteria; Cyano, Cyanobacteria; Plancto, Planctomycetes.
Figure 5.
Taxonomic composition of cyanobacterial and acidobacterial populations.
A) Family level taxonomic bins within the cyanobacterial sequences. The category environmental represents sequences most closely related to uncharacterized sequences from environmental surveys. B) Subdivision (SD) level taxonomic bins within the acidobacterial sequences. The category rare represents the sum total of subdivisions that individually accounted for <1% of acidobacterial sequences.