Table 1.
Primer sequences and their domain specific coverage rates.
Figure 1.
Percentage of non-coverage rates in 29 classified bacterial Phyla for the primers analyzed in this study.
Non coverage rates were calculated based on the coverage values in the SILVA SSU Ref 113 NR database, using SILVA Test Probe with zero mismatches allowed.
Table 2.
Overview of research parameters that were used to measure the phylogenetic information contained within short read sequences and the OTU richness calculated from each library.
Figure 2.
The Pearson Correlation between corresponding patristic distances in trees generated from nearly full length (x-axis) and short read libraries (y-axis) for the different primers investigated.
Patristic distances were normalized to a maximum value of one prior to plotting.
Table 3.
Overview of the research parameters that were applied in comparisons of short read and nearly full length sequence libraries.
Figure 3.
The result of the sliding window analysis on the nearly full length alignment.
The size of the window was 280 bp, conform the length of the short read sequences. Plus signs indicate the points that cover the regions sequenced by the primers studied.
Figure 4.
The Pearson Correlation between corresponding pairwise distances in trees generated from nearly full length (x-axis) and short read libraries (y-axis) for the different primers investigated.
Pairwise distances were normalized to a maximum value of one prior to plotting.
Table 4.
Taxonomic assignment performance of short read sequences.