Figure 1.
Localization of adiponectin in aorta.
A, Dual-immunofluorescence was performed in wild-type (WT) and adiponectin knockout (Adipo-KO) mice as described in Materials and Methods. B, High magnification images of aortic intima using confocal laser microscope were obtained from WT mice. Green, adiponectin; blue, DAPI; red, CD31.
Figure 2.
Existence of adiponectin protein in aortic intima.
Mice were perfused with cold saline to eliminate the contamination of circulating adiponectin. After removing perivascular fat, thoracic aorta was cut through and then intima was carefully shaved from the aorta. A, Western blotting with antibodies against adiponectin, perilipin A, and CD31 in aortic intima. B, Multimeric complexes of adiponectin protein in aortic intima. C, The mRNA level of adiponectin in fat tissue and aortic intima. WT, wild-type mice; KO, adiponectin knockout mice; N.D., not detected.
Figure 3.
Effect of LPS on endothelial adhesion molecules in aortic intima.
Aortic intima of wild-type (WT) or adiponectin knockout (Adipo-KO) mice were removed at 4 hours after LPS administration and were subjected to RT-PCR (A) and western blotting (B). Values are mean ± SEM; n = 6 for each group. * P<0.05, compared with the values of WT mice with saline treatment; ‡ P<0.05, compared with the values of WT mice with LPS administration group.