Table 1.
Viruses used to determine analytical specificity of the triplex RT-PCR assay.
Table 2.
Primer and probes used in the triplex RT-PCR.
Figure 1.
Analytical sensitivity of RT-PCR for singleplex, duplex and triplex assays for CSFV and ASFV detection.
Serial dilutions of A) in vitro transcribed CSFV RNA or B) linearised ASFV plasmid DNA were amplified in RT-PCRs containing primers and probes to detect either single, duplex or triplex targets.
Figure 2.
Analytical sensitivity of triplex and reference method PCRs on samples from experimentally infected animals.
(A) Viral RNA, extracted from blood samples taken at various time points post challenge from 2 animals (circles or squares) experimentally infected with CSFV and analysed by triplex RT-PCR (filled symbols) or CSFV RT-nPCR-TaqMan assay (open symbols). (B) Viral RNA, extracted from homogenised spleen samples taken from 6 pigs euthanized at various time points post challenge with ASFV and analysed by triplex RT-PCR (filled symbols) and ASFV-PCR (open symbols).
Table 3.
Comparison of triplex RT-PCR with reference RT-PCR and PCR assays for detection of CSFV and ASFV in experimental and field samples.
Table 4.
Diagnostic sensitivity and specificity estimates of triplex RT-PCR compared to reference RT-PCR and PCR assays for CSFV and ASFV.