Figure 1.
Length distribution of mappable counts of sequ-seqs type in two libraries of strawberry fruit.
Table 1.
Distribution of the small RNA sequences in the two libraries.
Figure 2.
Expression analysis of miRNAs in strawberry fruit stored at 20°C for 0 and 24 h by qRT-PCR.
The amount of expression was normalized by the level of actin 7 in qRT-PCR. All reactions of qRT-PCR were repeated three times for each sample. Left indicates the miRNA relative expression generated from the high-throughput sequencing; Right indicates the miRNA relative expression tested by qRT-PCR.
Table 2.
Differentially-expressed miRNAs in strawberry fruit stored at 20°C for 0 and 24 h. (More abundant were shown in Table S9).
Figure 3.
The T-plots of miRNA targets in the five different categories.
T-plots show the distribution of the degradome tags along the full-length of the target mRNA sequence. The red line represents the sliced target transcripts. (A) Example of the category 0 target gene03956−v1.0−hybrid for mdm−miR171a. (B) Example of the category 1 target gene07624−v1.0−hybrid for mtr−MIR1510a−p3_1ss5AT. (C) Example of the category 2 target gene05900−v1.0−hybrid for mdm−miR396b. (D) Example of the category 3 target gene18113−v1.0−hybrid for PC−5p−150653_24. (E) Example of the category 4 target gene04565−v1.0−hybrid for mdm−miR156b and gene01896−v1.0−hybrid for PC−5p−15285_237. The categories were based on the relative abundance of the tags at the target sites.
Table 3.
Identified miRNA targets involved in fruit senescence by degradome sequencing. (More targets were shown in Table S10).
Figure 4.
Putative molecular functions (A) and biological processes (B) of known and new candidate miRNA targets.
The GO analysis was carried out according to A. thaliana and O. sativa databases.