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Figure 1.

Flow sheet of patients, intervention and analysis.

Flow sheet of enrolled patients, intervention and analysis of data. IMCL = intramyocellular lipids.

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Figure 2.

Study protocol.

The clinical protocol included three visits and two periods. Period 1: weight maintaining diet, period 2: no physical activity, high fat diet (as additional fat snacks, 0.75 g fat/kgBW for 1.5 days prior to visit 3). Visit 1 Spiroergometry (calculation of 50% VO2max, trial on the treadmill). Visit 2 Two-step hyperinsulinaemic euglycaemic clamp using stable isotope technique. Visit 3 Assessment of intramyocellular (IMCL) and intrahepatocellular lipids (IHCL) before and after two hours aerobic exercise at 50% VO2max. Measurements of subcutaneous and visceral fat mass using MRI imaging.

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Figure 3.

Effect of aerobic exercise on intramyocellular (IMCL) and intrahepatocellular lipids (IHCL).

Figure 3a Individual IMCL and IHCL measurements before (pre) and after (post) a 2h-aerobic exercise at 50% VO2max of each subject. A significant decrease in IMCL (p<0.005) and a significant increase in IHCL (p<0.002) was documented. The diagram on the far right is a blow up of the IHCL data depicted in the middle part to show the changes in IHCL for volunteers with low IHCL levels. Figure 3b Changes (mean ± 1 SEM) of IMCL and IHCL (in % from baseline) following a 2h-aerobic exercise at 50% VO2max.

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Figure 4.

Effect of aerobic exercise on serum free fatty acid concentrations.

Individual (left) and overall (mean ± 1 SEM) levels of circulating FFA during the course of the 2 h-aerobic exercise at 50% VO2max.

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Table 1.

Clinical parameters, body composition and fat availability, exercise parameters, insulin sensitivity and MR-spectroscopy data in endurance trained athletes.

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